Michigan State University

Maris Laivenieks

J Gregory Zeikus

Claire Vieille

Pil Sung Kim

Anplasmid vector which provides a means to overexpress proteins in. The plasmid can be transformed efficiently by electroporation, and replicates in a stable manner in. The plasmid comprises at least one marker gene, operably linked to a first promoter functional in, an origin of replication functional in, a second promoter isolated from, and a cloning site downstream from the second promoter. Plasmids pLGZ901, pLGZ920, pLGZ921, and pLGZ922 are disclosed. The pckA gene polypeptide sequence and nucleic acid sequence of, including the promoter and ribosome binding site, is disclosed. Furthermore, a method for producing a recombinantis described, including a method of transformation. Additionally, a recombinantis disclosed and a method for producing succinate utilizing this recombinantis described.

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Anplasmid vector which provides a means to overexpress proteins inThe plasmid can be transformed efficiently by electroporation, and replicates in a stable manner inThe plasmid comprises at least one marker gene, operably linked to a first promoter functional inan origin of replication functional ina second promoter isolated fromand a cloning site downstream from the second promoter. Plasmids pLGZ901, pLGZ920, pLGZ921, and pLGZ922 are disclosed. The pckA gene polypeptide sequence and nucleic acid sequence ofincluding the promoter and ribosome binding site, is disclosed. Furthermore, a method for producing a recombinantis described, including a method of transformation. Additionally, a recombinantis disclosed and a method for producing succinate utilizing this recombinantis described.

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East Lansing, MI, United States of America

Maris Laivenieks