Evonik Degussa Gmbh

Evonik Operations Gmbh

Marion Schiebelhut

Thomas Haas

Steffen Schaffer

Mirja Wessel

Hans-Georg Joerg Hennemann

Markus Poetter

Frank Erhardt

Jasmin Gielen

Sabine Hafkemeyer

Katharina Berse

A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyzes one reaction of &#x3b2;-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyzes one of the reactions of the &#x3b2;-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof.

Process for the improved separation of a hydrophobic organic solution from an aqueous culture medium

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Haltern, Germany

Marion Schiebelhut