University of Massachusetts

University of California

Mario Stevenson

Tariq M Rana

Mark Sharkey

Akbar Ali

Robin Scott Nathans

Rajnish Kaushik

Hong Cao

Xiaonan Zhu

Disclosed herein, inter alia, are agents having antiviral activity and methods of use thereof.

Broad spectrum antiviral compounds and uses thereof

The invention is directed to a chimeric gammaretrovirus comprising an gammaretroviral virion which contains a lentiviral Vpx protein and methods of use thereof. In a particular aspect, the chimeric gammaretrovirus is a chimeric murine leukemia virus (MLV) comprising an MLV virion which contains a lentiviral Vpx protein. The invention is also directed to use of the chimeric gammaretrovirus to produce a MLV that can transduce a non-dividing cell (G1/S/G2), transduce a non-dividing cell; enhance the ability of a MLV to transduce a non-dividing cell; transduce a quiescent (G0) cell; and enhance the ability of a human immunodeficiency virus 1 (HIV-1) to transduce a quiescent (G0) cell.

Method of transducing non-dividing myeloid cells utilizing chimeric murine leukemia viruses containing Vpx

Provided herein are methods for detecting the presence of replication-competent HIV-1 virus in a subject who is being treated with an intensified highly active anti-retroviral therapy (HAART) regimen. These methods comprise selecting a subject who is being treated with an intensified HAART regimen including an integration inhibitor; obtaining a sample, e.g., a blood sample, from a subject; specifically amplifying a segment spanning two-long terminal repeat (2-LTR) junction of 2-LTR circles using PCR to determine the level of 2-LTR circles in the sample; and determining the presence of replication competent virus based on the level of 2-LTR circles in the sample. These methods can also be used to monitor an intensified HAART regimen by obtaining samples from the same subject at different time points during the HAART treatment, and comparing levels of the 2-LTR circles in those samples.

Methods of monitoring treatment effectiveness in HIV-infected patients receiveing intensified HAART regimens by measuring episomal 2-LTR circles

The present invention provides compounds and compositions for inhibiting Vif and methods for treating viral infection, e.g., HIV infection.

Composition and synthesis of new reagents for inhibition of HIV replication

The invention relates to a method of detecting a HIV-infected cell in a mammal. The method includes detecting an HIV 2-LTR circle DNA molecule obtained from a cell of an HIV-positive mammal, especially an HIV-1-positive human.

Detection of human immunodeficiency virus

The invention relates to methods of detecting a drug resistant HIV in a subject. The methods include detecting mutations associated with drug resistance in an HIV 2-LTR circle DNA molecule obtained from a cell of an HIV-positive subject, e.g., an HIV-1-positive human.

Detection of drug-resistant human immunodeficiency virus

The invention relates to methods of detecting a drug resistant HIV in a subject. The methods include detecting mutations associated with drug resistance in an HIV 2-LTR circle DNA molecule obtained from a cell of an HIV-positive subject, e.g, an HIV-1-positive human.

Growing community of inventors

Miami, FL, United States of America

Mario Stevenson