National Autonomous University of Mexico

The University of Arizona

Mario Soberon-Chavez

Alejandra Bravo-De-La-Parra

Maria Alejandra Bravo De La Parra

Liliana Pardo-Lopez

Bruce Elliot Tabashnik

Alejandra Bravo-De La Parra

Isabel Gomez-Gomez

Maria Alejandra Bravo-De La Parra

The present invention provides a method to obtain DNA constructs that encode 3-domain Cry toxins (also called Cry toxins, Bt toxins, or &#x3b2;-endotoxins) lacking helix &#x3b1;-1. These DNA constructs have been modified to encode proteins that kill insects that are resistant to the corresponding unmodified Cry toxins. The DNA constructs encoding the Modified 3-Domain Cry Toxins and the encoded Modified 3-Domain Cry Toxins are provided together with the molecular vectors and the host cell comprising said constructs and the recombinant methods to produce the Modified 3-Domain Cry Toxins. Additionally, compositions comprising the Modified 3-Domain Cry Toxins are disclosed. The resistance of the insects to unmodified Cry toxins is due to reduced toxin binding to the insects' midgut receptors. The invention further provides methods to overcome resistance in crop pests.

Suppression of resistance in insects to bacillus thuringiensis cry toxins that do not require the cadherin receptor

The invention provides nucleic acids obtained from strains ofencoding &#x3b4;-endotoxins having pesticidal activity against insect pests including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acid molecules encoding mutant pesticidal polypeptides, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid molecule of the invention. Such compositions find use in methods for controlling plant pests.

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Provided are pesticidal, particularly insecticidal, proteins which resemble S-layer proteins, as well as variants or mutants thereof, and DNAs encoding them. Further provided are methods and means for using said DNA or protein for controlling pests, particularly plant insect pests.

Bacterial proteins with pesticidal activity

Provided are pesticidal, particularly insecticidal, proteins which resemble S- layer proteins, as well as variants or mutants thereof, and DNAs encoding them. Further provided are methods and means for using said DNA or protein for controlling pests, particularly plant insect pests.

Growing community of inventors

Cuernavaca, Mexico

Mario Soberon-Chavez