The United States of America, as Represented by the Secretary, Department of Health and Human Services

University of Washington

Massachusetts Institute of Technology

University of Pittsburgh

Flowjo, LLC

Mario Roederer

Gary J Nabel

Peter D Kwong

John R Mascola

Tongqing Zhou

Zhi-yong Yang

Xueling Wu

Nicholas P Restifo

Mark Connors

Richard Wyatt

William Ray Schief

Yuxing Li

Carl-Magnus Hogerkorp

Luca Gattinoni

Alexander K Shalek

Michael D Stadnisky

Gilad Ofek

Robert Seder

Jiang Zhu

Enrico Lugli

Travis K Hughes

Lawrence Shapiro

Marc H Wadsworth, Ii

Margaret H Beddall

Pratip K Chattopadhyay

Joanne L Flynn

Patricia Darrah

William R Schief

Provided are methods of producing an isolated T memory stem cell population, the method comprising a) isolating naïve T cells from a mammal, wherein the mammal is not a mouse; b) activating the naïve T cells and expanding the numbers of naïve T cells in the presence of one or more non-specific T cell stimuli, one or more cytokines, and a GSK-3beta inhibitor. Also provided are methods of producing an isolated T memory stem cell population, the method comprising a) isolating lymphocytes from a mammal; b) sorting the lymphocytes using flow cytometry into a population comprising a phenotype comprising i) CD95+, CD45RO−, and CCR7+; and ii) CD62L+ or one or more of CD27+, CD28+, CD45RA+, and CD127+ to produce an isolated T memory stem cell population. Further embodiments of the invention provide related cells, populations of cells, pharmaceutical compositions, and methods of treating or preventing cancer.

Methods of producing T memory stem cell populations

Provided herein are compositions and methods for therapeutic and/or prophylactic treatment of an intracellular bacterial infection in a subject in need thereof, comprising one or more modulating agents, wherein the one or more modulating agents increase expression of IFNγ, IL-2, TNF, and/or IL-17 in systemic and/or lung T cells. In some embodiments, the increase of expression of IFNγ, IL-2, TNF, and/or IL-17 occurs in lung T cells. The lung T cells can be lung resident T cells or systemic T cells that are recruited to the lung. In some embodiments, the T cells are CD4+ and/or CD8+ T cells. In some embodiments, the intracellular bacterial infection is a(MTB) infection.

Compositions and methods for treating bacterial infections

Some embodiments of the methods provided herein relate to sample analysis and particle characterization methods for large, multi-parameter data sets. Frequency difference gating compares at least two different data sets to identify regions in a multivariate space where a frequency of events from a first data set is different than a frequency of events from the second data set according to a defined threshold.

Visualization, comparative analysis, and automated difference detection for large multi-parameter data sets

Provided are methods of producing an isolated T memory stem cell population, the method comprising a) isolating na&#xef;ve T cells from a mammal, wherein the mammal is not a mouse; b) activating the na&#xef;ve T cells and expanding the numbers of na&#xef;ve T cells in the presence of one or more non-specific T cell stimuli, one or more cytokines, and a GSK-3beta inhibitor. Also provided are methods of producing an isolated T memory stem cell population, the method comprising a) isolating lymphocytes from a mammal; b) sorting the lymphocytes using flow cytometry into a population comprising a phenotype comprising i) CD95+, CD45RO&#x2212;, and CCR7+; and ii) CD62L+ or one or more of CD27+, CD28+, CD45RA+, and CD127+ to produce an isolated T memory stem cell population. Further embodiments of the invention provide related cells, populations of cells, pharmaceutical compositions, and methods of treating or preventing cancer.

Provided are methods of producing an isolated T memory stem cell population, the method comprising a) isolating nave T cells from a mammal, wherein the mammal is not a mouse; b) activating the nave T cells and expanding the numbers of nave T cells in the presence of one or more non-specific T cell stimuli, one or more cytokines, and a GSK-3beta inhibitor. Also provided are methods of producing an isolated T memory stem cell population, the method comprising a) isolating lymphocytes from a mammal; b) sorting the lymphocytes using flow cytometry into a population comprising a phenotype comprising i) CD95+, CD45RO&#x2212;, and CCR7+; and ii) CD62L+ or one or more of CD27+, CD28+, CD45RA+, and CD127+ to produce an isolated T memory stem cell population. Further embodiments of the invention provide related cells, populations of cells, pharmaceutical compositions, and methods of treating or preventing cancer.

Monoclonal neutralizing antibodies are disclosed that specifically bind to the CD4 binding site of HIV-1 gp120. Monoclonal neutralizing antibodies also are disclosed that specifically bind to HIV-1 gp41. The identification of these antibodies, and the use of these antibodies are also disclosed. Methods are also provided for enhancing the binding and neutralizing activity of any antibody using epitope scaffold probes.

Neutralizing antibodies to HIV-1 and their use

A system is disclosed for thawing a frozen specimen that includes a cryovial containing a frozen specimen, a centrifuge tube containing a medium, and an adaptor for suspending the cryovial over the centrifuge tube in an inverted position, wherein the adaptor has an elongated tubular body defining opposed proximal and distal ends, and it has an axial bore extending from the distal end thereof to the proximal end thereof to define an outer periphery and an inner periphery, and wherein the outer periphery is dimensioned for insertion into the centrifuge tube and the inner periphery is dimensioned to receive the cryovial in an inverted position.

Adapter for suspending a cryovial over a centrifuge tube

Growing community of inventors

Washington, DC, United States of America

Mario Roederer