Washington University

University of Alberta

Instituto De Investigaciones Biotecnologicas- Instituto Tecnologico De Chascomus Consejo De Investigaciones Cientificas Y Tecnicas

Mario Feldman

Amirreza Faridmoayer

Maria Veronica Ielmini

Nancy Price

Fatima Garcia-Quintanilla

Jeremy Iwashkiw

Andrés Eduardo Ciocchini

The present application relates to glycoengineered outer membrane vesicles obtained from recombinant, gram-negative bacteria comprising hetereologous DNA encoding an enzyme or enzymes that produce a heterologous glycan that replaces all or a portion of the naturally-occurring O antigen in the lipopolysaccharides of the bacteria. Also provided by the present application are immunogenic compositions and vaccines prepared from the glycoengineered outer membrane vesicles expressing the heterologous glycans at their surface.

Glycoengineered outer membrane vesicles and use thereof as vaccines

Described herein are methods and systems for O-glycosylating proteins in vivo or in vitro in any prokaryotic organism. In these methods and systems, DNA comprising a gene that produces a PglL-like oligosaccharyltransferase and DNA comprising a gene that produces a protein to be O-glycosylated are used. The PglL-like oligosaccharyltransferase facilitates the covalent attachment of the glycan to the protein to produce the O-glycosylated protein. The methods and systems described herein provide an approach for the design and production of new vaccines and therapeutic agents for the treatment of various diseases.

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Growing community of inventors

Edmonton, Canada

Mario Feldman