Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Ludwig-maximilians-universitaet Muenchen

Harvard College

Ludwig-maximilians-universitat München

Marinus Huber

Ferenc Krausz

Mihaela Zigman Kohlmaier

Eric Mazur

Ioachim Pupeza

Marinna Madrid

Hanieh Fattahi

Joachim Pupeza

Valeria Nuzzo

Nabiha Saklayen

A particle analysis method and apparatus, including a spectrometry-based analysis of a fluid sample (), comprises the steps of creating a sample light beam S and a probe light beam P with a light source device () and periodically varying a relative phase between the sample and probe light beams S, P with a phase modulator device (), irradiating the fluid sample () with the sample light beam S, detecting the sample and probe light beams S, P with a detector device (), and providing a spectral response of the at least one particle (), wherein the light source device () comprises at least one broadband source, which has an emission spectrum covering a mid-infrared MIR frequency range, and the phase modulator device () varies the relative phase with a scanning period equal to or below the irradiation period of irradiating the at least one particle ().

Particle analysis method and apparatus for a spectrometry-based particle analysis

A method of measuring a polarization response of a sample (), in particular a biological sample, comprises the steps of generating a sequence of excitation waves (), irradiating the sample () with the sequence of excitation waves (), including an interaction of the excitation waves () with the sample (), so that a sequence of sample waves () is generated each including a superposition of a sample main pulse and a sample global molecular fingerprint (GMF) wave (E(t)), irradiating a reference sample (A) with the sequence of excitation waves (), including an interaction of the excitation waves () with the reference sample (A), so that a sequence of reference waves (A) is generated each including a superposition of a reference main pulse and a reference GMF wave (E(t)), optically separating a difference of the sample waves () and reference waves (A) from GMF wave contributions which are common to both of the sample waves () and reference waves (A) in space and/or time, and detecting the difference of the sample waves () and the reference waves (A) and determining a temporal amplitude of differential molecular fingerprint (dMF) waves (&#x394;E) () each comprising the difference of the sample and reference GMF waves. Furthermore, as a spectroscopic apparatus for measuring a polarization response of a sample () is described.

Methods and devices for measuring changes in the polarization response of a sample by field-resolved vibrational spectroscopy

In one aspect, a method of cell processing is disclosed, which includes disposing a plurality of cells on a substrate across which a plurality of projections are distributed and an electrically conductive layer at least partially coating said projections, exposing the cells to a cargo to be internalized by the cells, irradiating the substrate surface (and in particular the projections) with continuous wave or pulsed laser radiation. For example, one or more laser pulses having a pulse width in a range of about 1 ns to about 1000 ns can be applied so as to facilitate uptake of the cargo by at least a portion of the cells (e.g., the cells positioned in the vicinity of the projections (e.g., within hundreds of nanometer (such as less than 100 nm) of the projections)). In some embodiments, the laser pulses have a pulse width in a range of about 10 ns to about 500 ns, e.g., in a range of about 5 ns to about 50 ns.

Cellular poration using laser radiation

Growing community of inventors

Munich, Germany

Marinus Huber