Wisconsin Alumni Research Foundation

Marilyn L Bach

Fritz H Bach

Paul M Sondel

Michael J Sheehy

Rudolf Wank

A cellular method for typing lymphocyte defined human leukocyte antigens by utilizing human blood leukocytes which have been sensitized to proliferatively respond to the secondary stimulus of the leukocyte antigens to which they have been sensitized. The sensitization is accomplished by incubating, in vitro, in a mixed leukocyte culture, the leukocytes to be sensitized with leukocytes differing by only a single major histocompatibility complex haplotype or a single HLA-D antigenic determinant.

Method for typing human leukocyte antigens

A method for preparing a reagent for primed LD typing which comprises sensitizing purified human blood leukocytes to a secondary proliferative response by incubating them, in vitro, in mixed leukocyte culture, with lymphoblastoid cell lines from homozygous typing cells, or from other cells of specified genetic type homozygous for HLA-D, or other continuous line.

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Madison, WI, United States of America

Marilyn L Bach