Lubrizol Genetics, Inc.

Marilyn A Anderson

Adrienne Elizabeth Clarke

Edwina Cornish

Shaio-Lim Mau

Elizabeth G Williams

Shaio Lim Mau

Robert J Crawford

Philip J Harris

Geoffrey W Tregear

Rosslyn Hoggart

Robert Bernatzky

Hugh D Niall

DNA sequence of S-genes which encode S-proteins and control the self-incompatibility reaction in gametophytic self-incompatible plants have been identified. The DNA sequence encoding several S-proteins of N. alata and their attendant signal sequences are specifically provided. Regulatory sequences which direct expression of the S-genes in reproduction tissue of self-incompatible plants have also been identified. A method for the identification and isolation of cDNA and genomic DNA coding sequences of the S-genes is described.

Self-incompatibility gene

A class of proteins associated with self-incompatibility alleles of plants, preferably of gametophytic self-incompatibility type, are provided. These proteins inhibit pollen tube growth in a plant style having the associated self-incompatibility allele, and have defined amino acid homologies and cross-reactivities.

Self incompatibility protein

This invention provides an immunologic method for measuring plant pollen germination and pollen tube growth. This method is based on the finding that an antibody having specificity to .beta.-L-arabinofuranosyl residues binds to the surface of in vitro grown pollen tubes. Antibody binding can be measured qualitatively or quantitatively using a calibration curve. The immunoassay is useful for screening plants for response to substances that affect plant germination or pollen tube growth.

Pollen tube growth assay

Growing community of inventors

Sunbury, Australia

Marilyn A Anderson