Promega Corporation

Marie Schwinn

Keith V Wood

Lance P Encell

Monika G Wood

Thomas Machleidt

Andrew S Dixon

Thomas A Kirkland

Mary P Hall

Poncho Meisenheimer

Brock Binkowski

Matthew B Robers

Said A Goueli

James Unch

Melanie Dart

Nidhi Nath

Hicham Zegzouti

Subhanjan Mondal

Dileep K Pulukkunat

Kris Zimmerman

Lance Encell

Monika Wood

Brock F Binkowski

Said Goueli

Matthew Robers

Thomas Kirkland

Mary Hall

Keith Wood

Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Activation of bioluminescence by structural complementation

Activation of bioluminescene by structural complementation

Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex.

Internal protein tags

Disclosed herein are compositions and methods for stabilizing a luminogenic substrate such as coelenterazine or a functional analog thereof. The functional analog may be furimazine. The composition may include the luminogenic substrate, a thionucleoside, and an organic solvent, in which the thionucleoside is present in an amount effective to stabilize the luminogenic composition against decomposition. The method provided herein stabilizes the luminogenic substrate against decomposition by contacting the luminogenic substrate with an effective amount of the thionucleoside in the presence of the organic solvent. Also provided herein is a kit containing the composition.

Compositions and methods for stabilizing luminogenic substrates

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Madison, WI, United States of America

Marie Schwinn