Centre National De La Recherche Scientifique

UniversitÉ Paris Descartes

Institut De Recherche Pour Le Developpement (ird)

L'institut National De La Recherche Agronomique

Institut De Recherche Pour Le Developpement

Marie-Paule Lefranc

Martine Cerutti

Laurent Gauthier

Gerard Devauchelle

Marie-Alix Poul

Hassan Chaabihi

Michel Kaczorek

Celia Dechavanne

Francois Guillonneau

Jean-Michel Dugoujon

Florence Migot-Nabias

Célia Dechavanne

François Guillonneau

The present invention provides a method for determining the amino acid polymorphisms of heavy gamma chain of immunoglobulins G by a proteomic approach. This method distinguishes the immunoglobulins of the mother and those of the newborn in a blood sample obtained during the first months of the child's life. The invention also relates to the use of this method in the early diagnosis of vertically transmitted diseases in the neonate. The invention also provides peptides distinctive of G3m and IGHG3 alleles, and a kit comprising said peptides.

Method of neonatal serological diagnosis

Recombinant baculovirus used as an expression vector in the production of immunoglobulins with an insect cell. The invention is characterized by a first expression cassette comprising a first sequence coding for at least one portion of an immunoglobulin heavy chain, wherein the sequence is transcriptionally controlled by a first baculovirus promoter, and a second expression cassette comprising a second sequence coding for at least one portion of an immunoglobulin light chain, said second sequence being transcriptionally controlled by a second baculovirus promoter. The first and second promoters are two different promoters or derivatives of different promoters, the first and second promoters residing at different loci.

Recombinant baculovirus and use thereof in the production of monoclonal antibodies

Growing community of inventors

Clapiers, France

Marie-Paule Lefranc