Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Commissariat a L'energie Atomique

Institut National De La Sante Et De La Recherche Medicale

Centre Hospitalier Universitaire De Grenoble

Universite Jospeh Fourier

Université Grenoble Alpes

Université Joseph Fourier

Centre Hospitalier Universitaire De Grenoble Alpes

Marie-Line Cosnier

Patrice Caillat

Patrick Pouteau

François Berger

Myriam-Laure Cubizolles

Jean-Maxime Roux

Raymond Campagnolo

Franck Martin

Frédéric Revol-Cavalier

Magalie Faivre

Manuel Alessio

Remco Den Dulk

Ali Bouamrani

Nicolas Verplanck

Affif Zaccaria

Pascal Mailley

Dominique Lauro

Camille Laporte

Anubhav Bussooa

Magali Faivre

The invention relates to a device for transiently contacting at least one unit for capturing biological targets with a body fluid containing them, a method for recovering the captured targets for analysis, and a system for contacting and recovering a capture substrate included in said unit. The invention relates to samples obtained in particular in vivo from body fluids of the human body, e.g. circulating body fluids, said fluids possibly containing, as targets, proteins, oligonucleotides such as RNA or DNA, antibodies, enzymes or cells. A contacting device () according to the invention comprises: &#x2014;a sampling tip () having a contacting end intended to be introduced into a medium containing said fluid, and&#x2014;the or each capturing unit which is bound to said end and which comprises a capture substrate () defining a target capturing surface covered with at least one biocompatible and porous crosslinked polymer layer () designed to retain the or each capture substrate and only to allow biological particles including targets smaller than a limiting size to pass through, in such a way that the or each capture substrate is recoverable after said contacting by dissolution of the or each layer.

Device for transiently contacting at least one unit for capturing biological targets with a fluid containing them, method for recovering the captured targets and system for contacting and recovery

The present invention relates to a method for assaying catalytic plasma enzymes, such as transaminases and NADH-dependent enzymes, in a sample of whole blood, by measuring, in a microfluidic chamber, the decrease in the fluorescence of NADH consumed during the enzymatic reactions catalyzed by the NADH-dependent enzymes.

Method for assaying plasma enzymes in whole blood

A device () to sample molecules of biological interest is described. The device () includes capture zones () whose developed surface area is much greater than the surface so that sampling by contact allows a sufficient quantity of molecules to be obtained to achieve reliable analysis, whilst being sized so that the device () is neither invasive nor aggressive. In particular, the device comprises tiered capture zones () located on a support () fabricated using microtechnology techniques; the capture zones () may advantageously be functionalised.

Device for contact molecular sampling

Growing community of inventors

Grenoble, France

Marie-Line Cosnier