Novo Nordisk A/s

Novozymes A/s

Novozyme A/s

Marianne Thellersen

Allan Svendsen

Jens Sigurd Okkels

Shamkant Anant Patkar

Kim Borch

Torben Vedel Borchert

Pia Van Der Zee

Jesper Vind

Torben Halkier

Claus Crone Fuglsang

Ib Groth Clausen

John C Royer

Henrik Bisgangrd-Frantzen

Steen Troels Jorgensen

Erik Gormsen

Titus Kretzschmar

Dorte Aaby Petersen

Henrik Lund

Henrik Bisgard-Frantzen

Arne Agerlin Olsen

Niels Munk

Peter Rosholm

The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.

Lipolytic enzymes

The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzime capable of removing a substantial amount of fatty matter a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.

The present invention relates to a method of preparing a variant of a parent lipolytic enzyme, comprising (a) subjecting a DNA sequence encoding the parent lipolytic enzyme to random mutagenesis, (b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and (c) screening for host cells expressing a mutated lipolytic enzyme which has a decreased dependance to calcium and/or an improved tolerance towards a detergent or a detergent component as compared to the parent lipolytic enzyme.

Method of preparing a variant of a lipolytic enzyme

The present invention relates to lipase variants which exhibit improved properties, detergent compositions comprising said lipase variants, DNA constructs coding for said lipase variants, and methods of making said lipase variants.

Lipase variants

Enzyme preparation comprising a modified enzyme

A variant of a parent .alpha.-amylase enzyme having an improved washing and/or dishwashing performance as compared to the parent enzyme, wherein one or more amino acid residues of the parent enzyme have been replaced by a different amino acid residue and/or wherein one or more amino acid residues of the parent .alpha.-amylase have been deleted and/or wherein one or more amino acid residues have been added to the parent .alpha.-amylase enzyme, provided that the variant is different from one in which the methionine residue in position 197 of a parent B. licheniformis .alpha.-amylase has been replaced by alanine or threonine, as the only modification being made. The variant may be used for washing and dishwashing.

Amylase variants

Growing community of inventors

Frederiksberg, Denmark

Marianne Thellersen