The United States of America, as Represented by the Secretary, Department of Health and Human Services

Kite Pharma, Inc.

Wilson Wolf Manufacturing Corporation

Marianna Sabatino

Steven A Rosenberg

John R Wilson

Nicholas P Restifo

Robert L Somerville

Mark E Dudley

Arianne Perez

Jianjian Jin

David Stroncek

Provided herein are methods for delaying or inhibiting T cell maturation or differentiation in vitro for a T cell therapy, comprising contacting one or more T cells from a subject in need of a T cell therapy with an AKT inhibitor and at least one of exogenous Interleukin-7 (IL-7) and exogenous Interleukin-15 (IL-15), wherein the resulting T cells exhibit delayed maturation or differentiation. In some embodiments, the method further comprises administering the one or more T cells to a subject in need of a T cell therapy.

Methods of preparing T cells for T cell therapy

An embodiment of the invention provides a method of promoting regression of cancer in a mammal comprising obtaining a tumor tissue sample from the mammal; culturing the tumor tissue sample in a first gas permeable container containing cell medium therein; obtaining tumor infiltrating lymphocytes (TIL) from the tumor tissue sample; expanding the number of TIL in a second gas permeable container containing cell medium therein using irradiated allogeneic feeder cells and/or irradiated autologous feeder cells; and administering the expanded number of TIL to the mammal. Methods of obtaining an expanded number of TIL from a mammal for adoptive cell immunotherapy are also provided.

Methods of growing tumor infiltrating lymphocytes in gas-permeable containers

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Los Angeles, CA, United States of America

Marianna Sabatino