Children's Medical Center Corporation

Other

Institut Swisse De Recherches Experimentales Sur Le Cancer

Marian R Neutra

Jean-Pierre Kraehenbuhl

Helen M Amerongen

Frank A Robey

Pamela Kozlowski

Andreas Frey

David Prashker, Legal Representative

Winfried Weissenhorn

Nicholas Mantis

The present invention provides a fusion protein construct (gp41HA) consisting of the ectodomain of the HIV-1envelope glycoprotein gp41 fused to a fragment of the influenza virus HA2 hemagglutinin protein. Immunization in-vivo via an intraperitoneal prime followed by intranasal or intragastric boosts with gp41HA induces high concentrations of serum IgG antibodies and fecal IgA antibodies that reacted with gp41 in HIV-1viral lysate and are cross-reactive with gp41 in HIV-1lysate. Followup analyses by indirect immunofluorescence showed that both serum IgG and fecal IgA recognized human peripheral blood mononuclear cells infected with either syncytium-inducing (SI) or non-syncytium-inducing (NSI) North American HIV-1 field isolates, but not uninfected cells.

Fusion protein construct and method for inducing HIV-specific serum IgG and secretory IgA antibodies in-vivo

A method for generating antigen-sensitized Ig-A-producing lymphoblasts in a mammal, using an immunogen comprising an antigen or antigen mixture in association with hydroxylated calcium phosphate (hydroxy apatite) is administered to a mucosal surface of the mammal.

Hydroxyapatite-antigen conjugates and methods for generating a poly-Ig immune response

The present invention is a spatially aligned conjugated composition which comprises at least one chemically modified substance which is immunologically representative of a prechosen infectious agent and provides a chemical constituent for entering into and forming a thioether bond; a plurality of chemically substituted metallic oxide particles which range from about 10-10,000 nanometers and are able to enter into a thioether bond and covalent linkage; and at least one thioether bond and linkage joining the metallic oxide particles in a controlled and spatially aligned manner to the antigen or hapten. The conjugated composition may be alternatively employed as an immunogen; as a vaccine; as a diagnostic tool and reactant; and as an analytical material suitable for testing the pharmacological activity of new compounds.

Spatially aligned conjugated composition having a thioether bond linkage

Hydroxyapatite-antigen conjugates and methods for generating a poly-Ig

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Sherborn, MA, United States of America

Marian R Neutra