Novozymes, Inc.

Other

Cognis Corporation

Novozymes Biotech, Inc

Novozymes A/s

Japan Tobacco Inc.

Cognis IP Management Gmbh

Novozymes Biopharma Dk A/s

Maria Tang

Alan P Sloma

Regine Behr

David Sternberg

Stephen H Brown

Martin Anthony Gleeson

William R Widner

Krishna M Madduri

David L Craft

C Ron Wilson

L Dudley Eirich

John C Loper

Alfred A Brenner

Cathy A Cornett

Mark Eshoo

Linda Sternberg, Legal Representative

Kimberly M Brown

Alexander Blinkovsky

Alan V Klotz

Tony S Byun

Mikio Fujii

Chigusa Marumoto

Leslie Naggiar, Legal Representative

Randy M Berka

Lene Venke Kofod

Barbara Cherry

Michelle Maranta

Stephen Brown

William Widner

The present invention relates to methods of obtaining genetic competence in non-competentcells for their transformation with exogenous DNA.

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The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating ahost cell under conditions suitable for production of the hyaluronic acid, wherein thehost cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase.

Methods for producing hyaluronan in a recombinant host cell

The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parentcell under conditions conducive for the production of the heterologous biological substance, wherein (i) the mutant cell comprises a first nucleic acid sequence directing synthesis of the heterologous biological substance and a second nucleic acid sequence comprising a modification of at least one of the genes cypX and yvmC, which are involved in the production of a red pigment, and (ii) the mutant cell is deficient in the production of the red pigment compared to the parentcell when cultivated under the same conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to mutants ofcells and methods for producing the mutants.

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the &#x3c9;-hydroxylase complex of20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

The present invention relates to isolated polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

Polypeptides having aminopeptidase activity and nucleic acids encoding same

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the &ohgr;-hydroxylase complex of,20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

Cytochrome P450 monooxygenase and NADPH cytochrome P450 oxidoreductase genes and proteins related to the omega hydroxylase complex of Candida tropicalis and methods relating thereto

Growing community of inventors

Fairfield, CA, United States of America

Maria Tang