Hoechst Aktiengesellschaft

Forschungszentrum Juelich Gmbh

Nutrinova Nutrition Specialties & Food Ingredients Gmbh

Maria Regina Kula

Lothar Elling

Thomas Kiy

Astrid Zervosen

Werner Hummel

Rudiger Marquardt

Wolfgang Piepersberg

Brigitte Horsch

Brigitte Hoersch

Ruediger Marquardt

Andreas Seiffert-Stoeriko

Andreas Seiffert-Storiko

Frank Boermann

Juergen Distler

Stefan Verseck

Andreas Stein

The present invention relates to nucleotide-sugar-synthesizing enzymes (enzymes with nucleotidyltransferase or pyrophosphorylase activity) from nonparasitic protists, to a process for the preparation thereof and to the use thereof for preparing nucleotide-sugars. The enzymes according to the invention make possible or greatly simplify the enzymatic preparation of various nucleotide-sugars on the industrial scale from low-cost precursors. It is possible with the aid of the discovered enzymes to prepare, for example, GDP-fucose, GDP-mannose, UDP-glucose, UDP-glucosamine, UDP-galactose, UDP-galactosamine, UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine in economic quantities.

Nucleotide-sugar-synthesizing enzymes from nonparasitic protists

UDP-glucose pyro phosphorylase enzymes from nonparasitic protozoa

Method for the enzymatic galactosylation of monosaccharides and

The present invention relates to processes for the enzymatic synthesis of nucleotide-6-deoxy-D-xylo-4-hexuloses starting from a nucleoside monophosphate (NMP). These processes comprise simultaneous incubation of the following substances in a buffer solution:

Process for the synthesis of nucleotide-6-deoxy-D-xylo-4-hexuloses

A process for the enzymatic preparation of acetoin, particularly (+)-acetoin, employs a diacetyl reductase which can be obtained from a yeast or Lactobacillus strain.

Microbiologically-prepared diacetyl reductase

Growing community of inventors

Niederzier-Hambach, Germany

Maria Regina Kula