Genencor International, Inc.

E.i. Dupont De Nemours and Company

Genecor International, Inc.

Maria R Diaz-Torres

Nigel S Dunn-Coleman

Mark Scott Payne

Volker Schellenberger

Donald Eugene Trimbur

Matthew W Chase

Vasantha Nagarajan

Gregory Marshall Whited

Stephen K Picataggio

Olga V Selifonova

Sharon Loretta Haynie

Charles Edwin Nakamura

Ramesh V Nair

Anthony Arthur Gatenby

Thomas B Morrison

Brian S Miller

Amy Kuang-Hua Hsu

Richard D La Reau

Richard D Lareau

Stephen Kenneth Picataggio

Edwin W Lee

Described herein are methods and compositions for the production and secretion of polypeptides. Included herein is the use of interrupting peptide transport activity for an increase in polypeptide production and/or secretion.

Production of secreted polypeptides

The present invention provides a microorganism for the production of 1,3-propanediol from a variety of carbon sources in an organism capable of 1,3-propanediol production and comprising a) at least one gene encoding a dehydratase activity; b) at least one gene encoding a glycerol-3-phosphatase; and c) at least one gene encoding protein X. The protein X may be derived from aorgene cluster. The recombinant microorganism may further comprise d) at least one gene encoding a protein having at least 50% similarity to a protein selected from the group consisting of protein 1 (SEQ ID NO:60 or SEQ ID NO:61), of protein 2 (SEQ ID NO:62 or SEQ ID NO:63) and of protein 3 (SEQ ID NO:64 or SEQ ID NO:65) fromor

Method for the recombinant production of 1,3-propanediol

The present invention relates to the formate transport system in gram-positive microorganisms and provides methods for the production of products in a gram-positive microorganism. The present invention also provides the nucleic acid and amino acid sequences of FTAP 1 and FTAP 2 which are associated with formate transport.

Enhancing growth in gram-positive microorganisms using formate supplementation and inactivation of formate-associated transport proteins

The present invention provides an improved method for the production of 1,3-propanediol from a variety of carbon sources in an organism capable of 1,3-propanediol production and comprising DNA encoding protein X of a microorganismal dehydratase or protein X in combination with at least one of protein 1, protein 2 and protein 3, which proteins are comparable to those encoded by orfY, orfX and orfW, respectively from a microorganismal dha regulon. The protein X may be isolated from a diol dehydratase or a glycerol dehydratase. The present invention also provides host cells comprising protein X that are capable of increased production of 1,3-propanediol.

Recombinant organisms are provided comprising genes encoding glycerol-3-phosphate dehydrogenase, glycerol-3-phosphatase, glycerol dehydratase and 1,3-propanediol oxidoreductase activites useful for the production of 1,3-propanediol from a variety of carbon substrates.

Method for the production of 1,3-propanediol by recombinant

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Los Gatos, CA, United States of America

Maria R Diaz-Torres