Empresa Brasileira De Pesquisa Agropecuaria-embrapa

Universidade Federal Do Rio De Janeiro

Maria Fatima Grossi De Sa

Maria Cristina Mattar Da Silva

Thales Lima Rocha

Mariana Torquato Quezado De Magalhaes

Rafael Perseguini Del Sarto

Fernanda Pinheiro Da Cruz Waltenberg

Marcio Alves Ferreira

Eduardo Romano De Campos Pinto

Gustavo Ramos De Oliveira

The present invention relates to the field of insect pests control, using methods and compositions which comprise of alpha-amylase analogous mutant inhibitors (&#x3b1;AIs). More specifically, the invention provides new &#x3b1;AIs analogous mutant molecules for controlling insect pests, in particular boll weevils (), partially or totally presenting reduction of the amylolytic activity of the digestive enzymes in the intestinal lumen of the insect. Other aspects of the invention include gene constructs containing the nucleic acid molecules that code for the alpha-amylase inhibitors, heterologous expression methods of the new molecules in the active form, and the use of these molecules for controlling insect pests. The invention also provides analogous synthetic genes optimized for their transformation and expression in plants.

Alpha amylase mutant inhibitors isolated from phaseolus vulgaris with properties of controlling insect pests, compositions containing such mutants, and method of using thereof

The proposed innovation relates to the biotechnological improvement of plant species of commercial interest. More specifically, the present invention relates to the production of transgenic plants biotechnological with greater tolerance to water deficit and salt stress by means of the expression of a new gene of coffee (of thespecies), belonging to the HD-Zip family, and characterized by a homeodomain associated with a leucine zipper. The expression of this transcriptional factor is induced in leaves and roots of coffee plants subjected to various water deficit conditions (both moderate and severe); transgenic plants that over-express this gene inhibiting greater tolerance both to differ drought intensities and to high salt concentrations.

Use of the coffee homeobox gene CAHB12 to produce transgenic plants with greater tolerance to water scarcity and salt stress

The present invention belongs to an insecticide compound derived from a strain ofand refers to the field of controlling plant pests, particularly to the control of the boll weevil&#x2014;. More specifically, the object of the invention refers to a new gene for the new delta-endotoxin designated Cry8Ha and to the cloning and expression of the gene that encodes for the protein Cry8Ha in. There is provided the nucleotide sequence and encoding protein of the new delta-endotoxin, recombinant vectors and host cells. There is also provided processes and means for recombinant production and the use of the new delta-endotoxin for application in the control of the boll weevil. Additionally, the invention also provides an optimized synthetic gene for expression in cotton plants. Using the gene described herein, it is possible to transform plants based on techniques known by specialists in the art, for the expression of the endotoxin active against the boll weevil.

Genetic construct expressing insecticidal toxin and the method of using thereof

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Brasilia, Brazil

Maria Fatima Grossi De Sa