Amersham Life Science, Inc.

Maria Davis

Joseph Szasz

Carl Williams Fuller

Frank T Robb

Joseph A Mamone

Kimberly M Borges

R Bruce Moffett

Dan Sha

John J Cunniff

Enzymatically active thermostable alkaline phosphatases from Rhodothermus marinus, Thermus thermophilus, and Thermosipho africanus.

Thermostable alkaline phosphatases

An enzymatically active DNA polymerase having between 540 and 582 amino acids having a tyrosine at a position equivalent to position 667 of Taq DNA polymerase, wherein said polymerase lacks 5' to 3' exonuclease activity, and wherein said polymerase has at least 95% homology in its amino acid sequence to the DNA polymerase of Thermus aquaticus, Thermus flavus or Thermus thermophilus, and wherein said polymerase forms a single polypeptide band on an SDS PAGE.

Thermostable DNA polymerases

An enzymatically active DNA polymerase or fragment thereof having at least 80% homology in its amino acid sequence to at least a contiguous 40 amino acid sequence of the DNA polymerase of Thermoanaerobacter thermohydrosulfuricus.

Thermostable DNA polymerase from Thermoanaerobacter

Alkaline phosphatase from Thermus thermophilus has been isolated. The enzyme has a pH optimum of greater than 10.5 and is stable to heating at 65.degree. C for 1 hour. The claimed invention relates to a method of detecting nucleic acids in a sample by providing a nucleic acid probe labelled with thermostable alkaline phosphatase from Thermus thermophilus; contacting the sample with said labelled nucleic acid; and detecting said nucleic acid in said sample by means of said thermostable alkaline phosphatase.

Thermostable alkaline phosphatase of thermus thermophilus

Purified nucleic acid comprising a nucleotide base sequence selected from sequence ID NOS.: 1-8.

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Twinsburg, OH, United States of America

Maria Davis