Ospedale San Raffaele S.r.l.

Sangamo Therapeutics, Inc.

Sangamo Biosciences, Inc.

Fondazione Centro San Raffaele

Agc Biologics, Inc.

Fondazione Centro San Raffaele Del Monte Tabor

Molmed Spa

Maria Chiara Bonini

Michael C Holmes

Philip D Gregory

David Paschon

Lei Zhang

Luigi Naldini

Pietro Genovese

Attilio Bondanza

Zulma Magnani

Elena Provasi

Sara Mastaglio

Monica Casucci

Eliana Ruggiero

Luca Aldo Edoardo Vago

Fabio Ciceri

Zulma Irene Magnani

TCR AND PEPTIDES A T-cell receptor (TCR), which binds to a Wilms tumour 1 protein (WT1) peptide when presented by a major histocompatibility complex (MHC).

TCR and peptides

Disclosed herein are methods and compositions for inactivating TCR genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided. Disclosed herein are also methods and compositions for expressing a functional exogenous TCR in the absence of endogenous TCR expression in T lymphocytes, including lymphocytes with a central memory phenotype. Polynucleotides encoding exogenous TCR, vectors comprising polynucleotides encoding exogenous TCR and cells comprising polynucleotides encoding exogenous TCR and/or cells comprising exogenous TCR are also provided.

Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases

It is described in vitro methods for expanding, detecting or isolating rare populations of antigen specific memory T cells. It is also described an in vitro method for obtaining a genetically modified memory T cell population. Uses of cells so obtained are also disclosed.

Use of common gamma chain cytokines for the visualization, isolation and genetic modification of memory T lymphocytes

A chimeric antigen receptor (CAR) comprising an extracellular spacer which comprises at least part of the extracellular domain of human low affinity nerve growth factor (LNGFR) or a derivative thereof.

Chimeric antigen receptors

Disclosed herein are methods and compositions for modifying TCR genes, using nucleases (zinc finger nucleases or TAL nucleases) to modify TCR genes.

Use of common &#x3b3; chain cytokines for the visualization, isolation and genetic modification of memory T lymphocytes

Targeted disruption of T cell receptor genes using talens

Growing community of inventors

Peschiera Borromeo, Italy

Maria Chiara Bonini