Kyoto University

Mari Ohnuki

Shinya Yamanaka

Kazutoshi Takahashi

Michiyo Koyanagi

The present invention provides a method for screening for iPS cells exhibiting differentiation resistance using a marker identified as lincRNA or mRNA that is specifically expressed in an iPS cell line exhibiting differentiation resistance, and such markers.

Method for screening induced pluripotent stem cells

The present invention provides a method for selecting human induced pluripotent stem (iPS) cells which can be safely used for transplantation. That is, the present invention provides a method for selecting human iPS cells having reduced differentiation resistance, comprising the steps of: (1) inducing differentiation of human iPS cells; (2) detecting remaining undifferentiated cells after the step (1); and (3) selecting human iPS cells whose rate of remaining undifferentiated cells detected in step (2) is equivalent to or not more than that of control cells.

Method for selecting reduced differentiation resistance human induced pluripotent stem cells

Provided is a method of selecting highly safe pluripotent stem cells that do not exhibit differentiation resistance, comprising the steps of (1) inducing a pluripotent stem cell to differentiate, (2) culturing the cell under conditions for maintaining undifferentiated state, (3) detecting the generation of an undifferentiated cell by the cultivation, and comparing the finding with a control, and (4) selecting a pluripotent stem cell whose detected value is not more than a control generation value.

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Kyoto, Japan

Mari Ohnuki