Glycom A/s

Margit Pedersen

Manos Papadakis

Katrine Bych

Gyula Dekany

Elise Champion

Eric Samain

Pauline Peltier-Pain

Peter Becker

Ted Johanson

Katrine Bych Kampmann

The present invention relates to the field of recombinant production of biological molecules in host cells. More particularly it relates to a method for recombinant production of human milk oligosaccharides (HMO) using a genetically modified cell expressing a protein of the major facilitator superfamily (MFS).

HMO production

The invention relates to a genetically modified microorganism for making a oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source.

Oligosaccharide production

The present invention relates to the field of recombining production of biological molecules in host cells. The invention provides nucleic acid constructs that allow to modify expression of a desired gene using both in vitro and in vivo gene expression systems. The constructs can advantageously be used to produce a variety of biological molecules recombinantly in industrial scales, e.g. human milk oligosaccharides (HMO).

Nucleic acid construct for in vitro and in vivo gene expression

The invention relates to a microbial production of recombinant oligosaccharides, particularly of human milk oligosaccharides (HMOs), using a genetically modified microorganism, particularly, in antibiotic-free cultivating medium.

Growing community of inventors

Roskilde, Denmark

Margit Pedersen