Tsinghua University

Simpson Biotech Co., Ltd

China Medical University

Margaret Dah-Tsyr Chang

Chia-Chin Sheu

Shu-Chuan Lin

Wei-I Chou

Yuh-Ju Sun

Ping-Chiang Lyu

Shun-Lung Fang

Tan-chi Fan

Lin-Shien Fu

Hao-Teng Chang

Yuan-Chuan Lee

Chi-Shin Hwang

Rong-Yuan Huang

Shi-Hwei Liu

The present invention provides a method of determining and treating amyotrophic lateral sclerosis (ALS) in a subject, comprising the following steps: (1) measuring a concentration of an autoantibody against high mobility group box 1 protein (HMGB1) in a biological sample from the subject; and (2) administering an effective amount of ALS-treating drug to the subject whose measured concentration of the autoantibody against HMGB1 is higher than 0.874 &#x3bc;g/ml.

Method for determining and treating amyotrophic lateral sclerosis

The present invention relates to a recombinant protein comprising SEQ ID NO: 1 and a bacteria recognizing lectin. The present invention also relates to uses of the recombinant protein comprising detecting pathogens, removing endotoxins, determining the presence of an endotoxin or endotoxin-like material, and determining the presence of pathogen-associated molecular pattern (PAMP) comprising rhamnose-rhamnose (Rha-Rha), rhamnose-N-acetyl-mannosamine (Rha-ManNAc), N-acetyl-mannosamine-rhamnose (ManNAc-Rha), rhamnose-galatose (Rha-Gal), or galatose-rhamnose (Gal-Rha) in a sample, and use as a medicament, a disinfectant, a decontaminant, a surfactant or a diagnostic means. The present invention further relates to a method for prevention and/or treatment of conditions related to pathogen related infections in a patient in need thereof comprising: administering to said patient a pharmaceutically effective amount of composition comprising the recombinant protein, wherein the recombinant protein functions as an antagonist of PAMP comprising Rha-Rha, Rha-ManNAc, ManNAc-Rha, Rha-Gal, or Gal-Rha.

Recombinant bacteria recognizing protein and uses thereof

A method of treating or preventing inflammatory related diseases in a subject in need thereof comprising administering to said subject a pharmaceutically effective amount of a composition comprising an immune modulating polypeptide of SEQ ID NO: 1 is provided. A method for inhibiting cancer metastasis or growth of tumor in a subject in need thereof, comprising administering to the subject a pharmaceutically effective amount of a composition comprising an immune modulating polypeptide of SEQ ID NO: 1 is also provided.

Method of treating eosinophilia by administering an immunomodulating peptide

The present invention relates to an isolated peptide consisting of the amino acid sequence of SEQ ID NO: 1. The present invention also relates to a method for increasing expression of a target protein using the peptide consisting of the amino acid sequence of SEQ ID NO: 1, comprising (a) fusing the peptide with the target protein to form a recombinant protein; and (b) expressing the recombinant protein by an expression host.

Solubility enhancing peptide and use thereof

A polysaccharide-protein binding model of SBD, and a fibril-forming 14-residue peptide consisting of XNNNXXNYQXXXXXwherein the Xand Xmean a pair of opposite charged amino acid residues, and the X, X, X, X, X, or Xmeans an amino acid residue is described. A mixture for diminishing a polysaccharide, comprising at least two starch binding domains (SBDs) and a polysaccharide in a helix form is also presented. A method of providing an oligosaccharide, and a method of producing an amyloid-like fibril and use thereof are further described.

Polysaccharide-protein binding model and nano-fibril formation of a starch binding domain

The present invention provides a method and a system for increasing thermal stability of a target protein comprising fusing a starch binding protein (SBP) with the target protein to form a SBP-tagged recombinant protein and combining the SBP-tagged recombinant protein with a SBP-binding matrix. The present invention also provides a method for retaining an activity of a target protein in aquatic environment comprising fusing a starch binding protein (SBP) with the target protein to form a SBP-tagged recombinant protein and combining the SBP-tagged recombinant protein with a SBP-binding matrix.

Method for increasing thermal stability and retaining activity of a protein

The present invention provides a method for identifying starch binding sites of starch binding domain in CBM family. The CBM family is consisting of CBM20, CBM21, CBM25, CBM26, CBM34, and CBM41. The method further comprises predicting starch binding sites of starch binding domain in CBM family using the identified starch binding sites of starch binding domain with same topology.

Starch binding domain and use thereof

The present invention is for an isolated peptide consisting of the amino acid sequence of SEQ ID NO: 9 and using the peptide for treating asthma by reducing cytotoxicity of eosinophil derived toxins in bronchial epithelial cells of the subject suffers from asthma. The method comprises preparing a pharmaceutical composition having the peptide of SEQ ID NO: 9 and administering an effective amount of the composition to the subject.

Method of using heparin binding motif for treating asthma

A cell penetrating peptide which has following sequence: NYBXBXBNQX, wherein B represents a basic amino acid, Xrepresents an amino acid with an aromatic, a hydrophobic or an uncharged side chain, Xrepresents any amino acid, and Xrepresents N or none is described. A method for delivering a cargo into a subject by administrating a complex comprising the cell penetrating peptide and the desired cargo to the subject is also described.

Cell penetrating peptides for intracellular delivery

The present invention relates to an antibody mimetic of carbohydrate binding module (CBM) which specifically binds to an epitope on HIV glycoprotein. The present invention also relates to a method of detecting HIV glycoprotein.

Method for the detection of human immunodeficiency virus envelope (HIV) glycoprotein utilizing carbohydrate binding module (CBM) 20 or 21

A polysaccharide-protein binding model of SBD, and a fibril-forming 14-residue peptide consisting of X1NNNX2X3NYQX4X5X6X7X8, wherein the X1 and X8 mean a pair of opposite charged amino acid residues, and the X2, X3, X4, X5, X6, or X7 means an amino acid residue is described. A mixture for diminising a polysaccharide, comprising at least two starch binding domains (SBDs) and a polysaccharide in a helix form is also presented. A method of providing an oligosaccharide, and a method of producing an amyloid-like fibril and use thereof are further described.

A recombinant protein is prepared comprising a polypeptide of interest and a starch binding domain (SBD). The said SBD is obtainable from glucoamylase of fungi genus. The said recombinant protein comprising the said SBD can be purified by contacting with an affinity matrix such as starch, the SBD binds to the affinity matrix to isolate the recombinant protein. The recombinant protein can be purified by separating the association between the SBD and the affinity matrix by acid, alkaline, salt, or sugar. The polypeptide of interest may be an antibody, an antigen, a therapeutic compound, an enzyme, or a protein and may apply in pathogen destruction, vaccine producing, and oral care product manufacturing. The SBD further provides as a tool to screen or identify polysacchrides.

Recombinant protein comprising starch binding domain and use thereof

The present invention relates to a method for producing a protein, novel nucleic acid fragment, an expression vector, and a method for enhancing the protein production in The present invention provides that the combination of the modified signal peptide, increased copy number of the gene, and/or overexpressed Sec4p resulted in high level secretion of proteins.

Nucleic acids for enhancing gene expression and use thereof

A recombinant protein is prepared comprising a polypeptide of interest and a starch binding domain (SBD). The said SBD is obtainable from glucoamylase of fungi genusThe said recombinant protein comprising the said SBD can be purified by contacting with an affinity matrix such as starch, the SBD binds to the affinity matrix to isolate the recombinant protein. The recombinant protein can be purified by separating the association between the SBD and the affinity matrix by acid, alkaline, salt, or sugar. The polypeptide of interest may be an antibody, an antigen, a therapeutic compound, an enzyme, or a protein and may apply in pathogen destruction, vaccine producing, and oral care product manufacturing. The SBD further provides as a tool to screen or identify polysacchrides.

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Hsinchu, Taiwan

Margaret Dah-Tsyr Chang