Pioneer Hi-bred International, Inc.

Maren L Arling

William James Gordon-Kamm

Keith S Lowe

Ning Wang

George J Hoerster

Shiv Bahadur Tiwari

Alexandre Da Silva Conceição

Xinli Emily Wu

Carlos M La Rota

Theodore Mitchell Klein

Craig E Hastings

Ajith Anand

Todd J Jones

The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

Methods and compositions for rapid plant transformation

Growing community of inventors

Des Moines, IA, United States of America

Maren L Arling