Politecnico Di Milano

The State University of New York

The University of Arizona

Fondazione Cariplo

Biomimx S.r.l.

Marco Rasponi

Alberto Redaelli

Marvin J Slepian

Danny Bluestein

Paola Occhetta

A microfluidic device for controlled generation and/or culture and/or maturation of three-dimensional cells and/or tissue constructs that includes a culture chamber may include: a confinement apparatus configured to define at least one compartment configured to contain a cellular matrix and at least one compartment configured to contain a culture medium, the confinement apparatus being hydrophobic and pervious to the culture medium; and/or at least one counter element. The confinement apparatus and at least one counter element may be reciprocally mobile between resting and compression positions of the cellular matrix. A method for controlled generation and/or culture and/or maturation of three-dimensional cells and/or tissue constructs at a microscale may include: controlled compression of a cellular matrix for a predetermined period of time. The cellular matrix may be delimited by a confinement apparatus that is pervious to a culture medium.

Microfluidic devices and related methods for generation and/or culture and/or maturation of three-dimensional cells and/or tissue constructs

Provided herein is technology relating to microfluidic devices and particularly, but not exclusively, to devices, methods, systems, and kits for imparting stresses on a fluid flowing through a microfluidic device that is designed to mimic a stress profile of a macrofluidic device or pathology.

Methods, devices, and systems for microfluidic stress emulation

Growing community of inventors

Milan, Italy

Marco Rasponi