Novozymes A/s

Henkel Ag & Company, Kgaa

Marco Malten

Allan Svendsen

Jesper Vind

Thomas Agersten Poulsen

Kim Borch

Lise Munch Mikkelsen

Christian Lundager Gylstorff

Christian Jackel

Luise Erlandsen

Carsten Horslev Hansen

Hanne Philbert Nielsen

Svend Gunnar Kaasgaard

Padmavathi Balumuri

Vivek Srivastava

Vasudeva Prahlada Rao

Preethi Ramaiya

Shikha Gupta

Carsten Hørslev Hansen

Beth Nelson

Keith McFarland

Vibeke Skovgaard Nielsen

Astrid Benie

Dorte Marie Koefoed Klitgaard

Werner Besenmatter

Katarina Larson

Benie Astrid

Christian JÄCKEL

The present invention relates to detergent compositions comprising a variant of a parent lipase which variant has lipase activity, has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, and comprises substitutions at positions corresponding to T231R+N233R and at least one or more (e.g., several) of D96E, D111A, D254S, G163K, P256T, G91T, and G38A of SEQ ID NO: 2. The present invention also relates to use of the compositions, methods of producing the compositions, and methods of cleaning. The present invention furthermore relates to variants and polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides.

Detergent compositions

The present invention relates to lipase variants and compositions comprising (i) at least one surfactant and (ii) at least one lipase variant of the invention. Furthermore, the present invention relates to methods of using the compositions.

Lipase variants and compositions comprising surfactant and lipase variant

The present invention relates to a variant of a parent lipase, which parent lipase has the amino acid sequence of SEQ ID NO: 2, wherein the variant has lipase activity, comprises a substitution at a position corresponding to position 51 and comprises a substitution or remains unaltered at a position corresponding to position 96 of SEQ ID NO: 2. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Lipase variants and polynucleotides encoding same

The present invention relates to glucoamylase variants, polynucleotides encoding the variants, methods of producing the variants, and methods of using the variants. Also described are the use of glucoamylases of the invention for starch conversion to produce fermentation products, such as ethanol, and syrups, such as glucose. The invention also relates to a composition comprising a glucoamylase variant of the invention.

Glucoamylase variants and polynucleotides encoding same

The present invention relates to a composition comprising: (a) a source of sulfite; and (b) a lipase variant of a parent lipase, wherein said variant has improved stability towards sulfite as compared to the parent lipase.

Sulfite compositions

The present invention relates to glucoamylase variants having an increase in specific activity and/or an increase in melting temperature measured by TSA of at least 0.5 degrees Celsius, and further wherein the variants have at least at least 85%, at least 90%, at least 95% identity, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

The present invention relates to subtilisin variants and methods for obtaining subtilisin variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Subtilisin variants and polynucleotides encoding same

The present invention relates a method of obtaining a detergent composition comprising introducing (a) a lipase variant of a parent lipase which variant has at least 60% sequence identity with SEQ ID NO: 2, a substitution at a position corresponding to D254 of the mature polypeptide of SEQ ID NO: 2 and has lipase activity and (b) an anionic surfactant, wherein said composition has increased stability in comparison with a corresponding composition comprising the parent lipase.

Detergent compositions comprising lipase

Enzymes and/or polypeptides and/or mixtures of interest are evaluated during hydrolysis of cellulosic material by the use of indicator constituents such as fluorescent agents, resulting in efficient high-throughput analysis of enzymes and/or polypeptides. A high-throughput assay for the analysis of inter alia, pretreated corn stover (PCS) hydrolysis is also disclosed.

Method of analyzing cellulose decay in lignocellulosic material hydrolysis

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Copenhagen, Denmark

Marco Malten