Politecnico Di Milano

Fondazione Istituto Italiano Di Tecnologia

Universitá Degli Studi Di Genova

Ruths S.p.a.

Marco Castello

Alberto Diaspro

Paolo Bianchini

Giuseppe Vicidomini

Federica Villa

Alberto Tosi

Colin J R Sheppard

Giorgio Tortarolo

Mauro Buttafava

Simonluca Piazza

A method for operating a point laser-scanning microscope includes scanning a sample with a focused illumination laser beam; recording a plurality of images by detecting elements being configurable to an intensity mode, in which the recorded images are intensity images g(n) related to photons collected during an entire dwell time of the illumination beam on an individual position n, or to a time-resolved mode, in which the recorded images are time-resolved images g(n, t), the collected photons being discriminated based on their arrival times to individual detecting elements; calculating a fingerprint image a by summing the plurality of intensity images g(n) over all positions n; estimating shift matrices sand sfrom the intensity images g(n); reconstructing at least one of a time-resolved object function fand an intensity object function f; and visualizing at least one of a high-resolution time-resolved image fand a high-resolution intensity image f.

Time-resolved imaging method with high spatial resolution

A heat exchange unit, adapted to performing heat exchange between first and second fluids at different temperatures, comprises a heat exchange apparatus provided with a tube bundle, crossed internally by said first fluid, and containing means of the second fluid, adapted to receiving the exchange apparatus so that at least one portion of the tube bundle is externally surrounded by the second fluid. The exchange apparatus further comprises connection means which are structurally separate from said retainer means and to which one end of the tube bundle is operatively connected.

Heat exchange units

Growing community of inventors

Genoa, Italy

Marco Castello