L'oreal

Nestec S.a.

Societe L'oreal Sa

Marcelle Regnier

Rainer Schmidt

Catherine Mace

Markus Baur

Armand Malnoe

Andrea M A Pfeifer

Jean-Baptiste Galey

Laurent Marrot

Pascale Pelletier

Catherine Causse

Marie-Jeanne Staquet

Daniel Schmitt

The present invention relates to improved continuous (immortalized) cell lines, in particular keratinocytes and melanocytes derived from normal human skin tissue. The present invention also relates to novel serum-free media for isolating, producing and maintaining said improved continuous keratinocyte and melanocyte cell lines. The present invention also relates to methods for producing primary melanocytes and keratinocytes under serum-free conditions without any feeder cells.

Immortalized human skin cell lines and novel serum-free medium useful for the production thereof

A cosmetic or dermatological composition, comprising at least one extract of a rosacea of the genus Sanguisorba in a quantity of approximately 0.002% to approximately 10% by weight, based on the total weight of said composition.

Use of at least one extract of a rosacea of the genus Sanguisorba officinalis for promoting pigmentation of the skin and/or the body hair and/or the cranial hair

Methods for determining the sensitizing, irritant and/or allergenic potential of a candidate substrate are provided. These methods involve simultaneously cultivating keratinocytes and Langerhans cell precursors in a nutrient medium in order to effect differentiation of the Langerhans cell precursors, and contacting the candidate substrate with the cultured cells to determine the effect if any on markers associated with sensitizing, irritant or allergenic potential.

Evaluating the sensitizing/irritant/allergenic potential of a given

A cosmetic composition for depigmenting or bleaching mammalian skin or both, which contains:

Method of depigmenting or bleaching mammalian skin using

The present invention relates to a reconstructed skin model, characterized in that it comprises an epidermis equivalent on a support, the said epidermis equivalent comprising at least keratinocytes and at least induced or noninduced precursors of Langerhans' cells, as well as the process for preparing the said skin equivalent. The invention also relates to an epidermis equivalent, characterized in that it comprises at least keratinocytes and at least induced or noninduced precursors of Langerhans' cells and to the process for preparing it.

Skin equivalent comprising langerhans' cells

Growing community of inventors

Paris, France

Marcelle Regnier