Tecan Trading Ag

Tecan Ag

Marcel Rutishauser

Nikolaus Ingenhoven

Anton Posch

Remo Hoechli

Ralf Bartl

A gel electrophoresis cassette () is disclosed which includes two plates () and at least one seal () which separates these plates. The seal () is annular; it may be positioned essentially in the region of the outer edge of the plates (). For performing an electrophoresis in a second dimension following an isoelectric focusing in an first dimension, one of the plates () includes a recess () for inserting a strip holder () having a base plate (), which has a carrier surface () on which an IEF strip () is accommodated. The base plate () includes at least one stop () offset to a lower level in relation to the carrier surface () and a sealing surface (). The strip holder () is insertable into this recess () in such a way that the stop () of the base plate () is applied to the outer surface of the plate () and the sealing surface () presses tightly against the inner surface () of the recess (). In addition, systems and methods for performing a 2-D gel electrophoresis are disclosed.

Cassette, system, and 2-D gel electrophoresis method for separating molecules

A strip holder () is disclosed for accommodating a gel strip () for separating molecules using gel electrophoresis. The strip holder () disclosed is distinguished by including a baseplate (), at least one stop (), which is offset to a lower level in relation to the carrier surface (), and at least one sealing surface (), this stop () being implemented to be applied to counter surfaces () of an electrophoresis chamber, through which offset to a lower level of the stop () the installation depth of the strip holder () carrying a gel strip () into this electrophoresis chamber is determined and the sealing surface () ensuring a sealing installation of the strip holder () carrying a gel strip () into this electrophoresis chamber. Such a chamber () for isoelectric focusing of molecules in gel strips () is distinguished by including such a strip holder (), a frame (), and a cover (). Another chamber in the form of a cassette (), for performing an electrophoresis in the second dimension following the isoelectric focusing, includes two plates () and at least one seal () which separates these plates and is distinguished by including such a strip holder (), the strip holder being sealingly inserted into a recess () in one of these plates (). The first dimension of a corresponding 2-D gel electrophoresis method is also disclosed.

Strip holder, chamber, cassette, and 2-D gel electrophoresis method and system for performing this method for separating molecules

A holder ( ) for sample liquid containers, e.g. Eppendorf vessels ( ), is horizontally displaceable between a working position above a magnetic arrangement ( ) and a heating position above a hotplate ( ). The latter are supported on a baseplate ( ) which can be vertically displaced by means of a lifting device ( ) between a lowered passive position and a raised active position in which the magnetic arrangement ( ) and the hotplate ( ) act on the sample liquid in the Eppendorf vessels ( ) and attract ferromagnetic particles suspended there. To improve the interaction between the ferromagnetic particles and the sample liquid, the magnetic arrangement ( ) can be transversely moved by a stepping motor ( ) between a first position, in which, for example, a magnetic plate ( ) acts on a row of Eppendorf vessels ( ) from the right side, and a second position, in which said magnetic plate ( ) acts on the same row of Eppendorf vessels ( ) from the left side.

Growing community of inventors

Wolfhausen, Switzerland

Marcel Rutishauser