Hifibio Sas

Marcel Reichen

Raphael Clément Li-Ming Doineau

Arnaud Reitz

Sami Ellouze

Bingqing Shen

Annabelle Gerard

The invention relates to a method for ordering, sorting and/or focusing particles in a first microfluidic channel system, the method comprising the steps of i) providing for a first microfluidic channel comprising at least a first and a second inlet and a first outlet, ii) injecting a first fluid into the channel through said first inlet, iii) injecting a second fluid into the channel through said second inlet, wherein the viscosity of the first fluid is higher than the viscosity of the second fluid, such that the two fluids flow in a laminar fashion unmixed side by side, and one of the two fluids comprises the particles to be ordered, sorted and/or focused. The invention also relates to a microfluidic channel system for sorting different particles into one droplet.

Particle sorting in a microfluidic system

The present invention relates to a method for analyzing and selecting a specific droplet among a plurality of droplets (), comprising the following steps: &#x2014;providing a plurality of droplets (), &#x2014;for a droplet () among the plurality of droplets, measuring at least two optical signals, each optical signal being representative of a light intensity spatial distribution in the droplet for an associated wavelength channel, &#x2014;calculating a plurality of parameters from the optical signals, &#x2014;determining a sorting class for a droplet according to calculated parameters, &#x2014;sorting said droplet according to its sorting class, wherein the plurality of parameters comprises the coordinates of a maximum for each optical signal and a co-localization parameter and the at least two calculated parameters used for the determining step comprises the co-localization parameter.

Method for analyzing and selecting a specific droplet among a plurality of droplets and associated apparatus

The present invention concerns processes for barcoding nucleic acids from single cells and processes for genotyping single cells having a phenotype of interest.

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Waedenswil, Switzerland

Marcel Reichen