Dsm IP Assets B.v.

Dsm Fine Chemicals Austria Nfg Gmbh & Co. Kg

Marcel G Wubbolts

Petrus M M Nossin

Helmut Schwab

Wolfgang Skranc

Gerhard Steinbauer

Michael Stanek

Harald Pichler

Peter Pojarliev

Katrin Eppelmann

Micheal Stanek

Joannes Kierkels

Manuela Hermann

Susanne M Kremer

Christoph Zenzmaier

Petronella Catharina Raemakers-Franken

Paul M Brandts

Wijnand P H Peeters

Sandra Ernste

Stefaan M A Wildeman De

Martin Schuermann

Leon J R M Raeven

Process for biochemical synthesis of 1,4-butanediannine in a microorganism having an increased level of an ornithine decarboxylase activity as compared to the native level of the ornithine decarboxylase activity, in which the increased ornithine decarboxylase activity is obtained by overexpression of an ornithine decarboxylase encoding gene with increased translational and/or transcriptional efficiency. The process is performed by excreting 1,4-butanediamine produced in the microorganism into a fermentation broth, and recovering the 1,4-butanediannine from the fermentation broth. The increased translational and/or transcriptional efficiency is obtained by the use of a strong, regulated promoter consisting of an isopropvl-B-D-thiogalactoside (IPTG) inducible strong promoter.

Biochemical synthesis of 1,4-butanediamine

Polypeptide and recombinant protein having esterase activity which exhibit the amino acid sequence SEQ. ID. No. 1 and the use thereof.

Polypeptide having esterase activity and recombinant esterase and use thereof

The invention relates to a process for biochemical synthesis of 1,4-butanediamine in a microorganism having an increased level of an ornithine decarboxylase activity (increased ODC activity) as compared to the native level of the ornithine decarboxylase activity, wherein in the microorganism also an increased activity of N-acetylglutamate formation is present as compared to the native level of activity of N-acetylglutamate formation in the microorganism and wherein 1,4-butanediamine produced in the microorganism is excreted into a fermentation broth, and is recovered from the fermentation broth. The invention also relates to vectors, plasmids and hosts carrying a corresponding increased ODC activity and an increased activity of N-acetylglutamate formation.

Method of resolving stereoselectively racemic 2-alkyl-5-halopent-4-ene-carboxylic esters of the formula (I) 

The invention relates to biochemical synthesis of 6-amino caproic acid from 6-aminohex-2-enoic acid compound or from 6-amino-2-hydroxyhexanoic acid, by treatment with an enzyme having &#x3b1;,&#x3b2;-enoate reductase activity towards molecules containing an &#x3b1;,&#x3b2;-enoate group and a primary amino group. The invention also relates to processes for obtaining suitable genetically engineered cells for being used in such biotransformation process, and to precursor fermentation of 6-amino caproic acid from intermediates leading to 6-amino caproic acid. Finally, the invention relates to certain novel biochemically produced compounds, namely 6-aminohex-2-enoic acid, 6-aminohexanoic acid, as well as to caprolactam produced therefrom and to nylon-6 and other derivatives produced from such biochemically produced compounds or caprolactam.

Biochemical synthesis of 6-amino caproic acid

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Sittard, Netherlands

Marcel G Wubbolts