C-lecta Gmbh

New Matterhorn, LLC

Clariant Finance (bvi) Limited

Marc Struhalla

Gerd Reinhardt

Thomas Greiner-Stoeffele

Andreas Vogel

Claudia Feller

Rico Czaja

Birgit Brucher

Daniel Schwarze

Sabrina Koepke

Ramona Schmiedel

Hedda Merkens

Kamila Rzeznicka

Andreas Petri

Christopher David Bayer

Meike Hoffmann

Meike Ballschmitter

Thomas Greiner-STÖFFELE

Hans Jürgen Scholz

Sabrina Köpke

The present invention relates to a method for producing trehalose, comprising the steps of mixing and reacting, in any order, (i) at least one alpha-phosphorylase capable of catalyzing the production of alpha-D-glucose 1-phosphate intermediate from a saccharide raw material, and from at least one phosphorus source; (ii) at least one trehalose phosphorylase capable of catalyzing the production of trehalose from an alpha-D-glucose 1-phosphate intermediate and a glucose substrate, wherein the trehalose phosphorylase is a trehalose phosphorylase variant with an amino acid sequence which differs from the amino acid sequence of a wild type trehalose phosphorylase in at least one amino acid position, (iii) at least one saccharide raw material which produces an alpha-D-glucose 1-phosphate intermediate and a co-product by catalytic action of the alpha-phosphorylase; and (iv) at least one phosphorus source selected from the group consisting of a phosphoric acids and an inorganic salt thereof.

Method for producing trehalose employing a trehalose phosphorylase variant

The invention relates to ketoreductases and the use thereof. The ketoreductases of the invention are particularly useful for enzymatically catalyzing the reduction of ketones to chiral secondary alcohols.

Ketoreductases

An expression vector including two separately inducible converging promoters P1 and P2, and expression system including such an expression vector and an additional regulator vector, a method of protein expression using such an expression system, and a method of investigating (meta)genome libraries using such an expression system.

Expression vector

A method for generating a variant library of DNA sequences starting from at least one DNA starting sequence and including the steps: a) selecting at least two mutation sites in the starting sequence; b) dividing the DNA starting sequence into at least two sequence segments such that at least two of these sequence segments each contain at least one of the mutation sites; c) amplifying the sequence segments by polymerase chain reaction using at least five different oligonucleotides, where at (i) least one of the oligonucleotides can attach to each mutation site; (ii) at least two of the oligonucleotides can attach to at least one mutation site, and (iii) mutations are introduced, via mismatch positions, into the PCR amplificates by the oligonucleotides at the mutation sites where at least two mutations are introduced at at least one of the mutation sites; and d) linking the amplificates to give DNA sequences.

Process for generating a variant library of DNA sequences

The invention relates to the use of an amidohydrolase for preparing foodstuffs or stimulants.

Heat-stable asparaginase for reducing acrylamide in food stuffs or stimulants

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Leipzig, Germany

Marc Struhalla