Molecular Genetics Research and Development Limited Partnership

Molecular Genetics, Inc.

Marc S Collett

Anthony F Purchio

Tomas Kempe

William J DeLorbe

Methods and compositions are provided for the cloning and expression of Rift Valley Fever Virus genes in single cell host organisms. Also described are methods for culturing these novel single-cell organisms to produce Rift Valley Fever Virus gene gene products which may be formulated for use as products which may be formulated for use as immunogens in vaccines to protect against Rift Valley Fever Virus infections.

Vaccines against rift valley fever virus

Methods and compositions for the insertion and molecular cloning of RNA in DNA cloning vectors are described. RNA molecules to be cloned are modified by the ligation of oligonucleotide linkers onto the termini of the RNA molecule using T4 RNA ligase. Such linkers may be composed of DNA, RNA, or mixtures of each and facilitate the insertion and ligation of the RNA species into a DNA cloning vector. The resulting recombinant vectors are used to transform host cells which provide for the generation of multiple DNA copies of the RNA molecule.

Growing community of inventors

Minnetonka, MN, United States of America

Marc S Collett