Dako Denmark A/s

Other

Diagnostic Biosystems

Dako Corporation

Dakocytomation Denmark A/s

Marc Key

Marc E Key

John Favuzzi

Gordon Feingold

Rosanne Welcher

Kristopher Scott Buchanan

Benno M Guggenheimer

Bipin Gupta

Lars Winther

Bob Lathrop

Robert Lathrop

Loren Bland

James B Gilmartin

Mark Richard Holbrook

Michael J Barber

Robert Clark

Doug Sweet

Martin N Lindberg

David A Stanforth

Jeff Rau

Benno Guggenheimer

John Van Bosch

Uffe Lovborg

Jonathan R Sweda

Elizabeth Oliver Spaulding

Merritt M Martin, Jr

Dawn M Rohrbacker

Jeffery L Rau

Douglas F Sweet

Scott A Devore

Loren L Bland

Alfonso Heras

Sandy Krishnamurthy

An antigen protection method for enabling delayed sample processing may include: fixing a sample; embedding the sample; sectioning the sample and placing a section of the sample in contact with a microscope slide to produce a sectioned sample; de-embedding the sectioned sample; optionally rehydrating the sectioned sample; performing target retrieval on the sectioned sample; and optionally performing target visualization on the sectioned sample. The method may further include the steps of: applying a protecting reagent onto the sectioned sample; drying the protecting reagent; and waiting a period of time, such as greater than six hours, and these steps may be performed after the de-embedding step, rehydrating step, and/or target retrieval step.

Antigen protection method for enabling delayed sample processing

A sample protection method is provided which may be used for protecting a biological sample on a microscope slide, such as during heat-induced target retrieval and/or after heat-induced target retrieval such that: 1) the sample remains adherent to the microscope slide; and 2) the microscopic morphology of the biological sample remains intact. In some embodiments, the sample protection method may include the steps of: creating a sectioned sample that is in contact with a microscope slide; applying a protecting reagent onto a sectioned sample that is in contact with a microscope slide and drying the protecting reagent in which the protecting reagent may be both applied and dried onto the sectioned sample before and/or after performing target retrieval on the sectioned sample. The protecting reagent may include a water-soluble polymer and/or a water-soluble wax, such as polyethylene glycol, polypropylene glycol, polyvinyl alcohol, polyvinylpyrrolidone, alginic acid, and carrageenan.

Sample protection method

A deparaffinizing composition and method are provided for removing paraffin wax from biological samples. The deparaffinizing composition may include: a paraffin solvent that may be a first apolar paraffin solvent; a second apolar solvent that may be at least partially miscible with water; a first polar solvent that may be miscible with both water and apolar solvents; a second polar solvent that may be water; and a detergent. The deparaffinizing method may include the steps of: incubating the sample for a time period in a deparaffinizing composition; and rinsing the sample with an aqueous buffer. Preferably, the step of incubating the sample for a time period in the deparaffinizing composition may include heating the sample and the composition to between approximately 35 to 65 degrees Celsius.

Deparaffinizing compositions and methods

The present disclosure relates to counterstains for staining a biological sample on a single slide in preparation for microscopic examination. The counterstains are used to analyze the sample on the single slide using both brightfield and fluorescent illumination. The counterstains can identify both morphological details and molecular structures within the cells contained in the sample. The counterstains can be used in conjunction with other molecular stains.

Counterstains for a biological sample

The present disclosure relates to an aqueous sealing fluid for sealing a stained biological specimen present on a microscope slide for microscopic analysis. The sealing fluid protects the biological specimen from drying artifacts, protects stains performed on biological specimen from fading, and protects stains performed on biological specimens from being dissolved by organic mounting media. Methods of using the aqueous sealing fluid are also disclosed.

Sealing fluid for microfluidic analyses

A method for labeling concentration density differentials of an analyte in a biological sample is provided. The method may including the steps of: binding an enzyme to an analyte contained in a sample, the enzyme capable of acting on at least two chromogens; incubating the sample with a first chromogen for a first time period to generate a first color chromogen-enzyme product, the first color chromogen-enzyme product reflecting light observable as a first color; and incubating the sample with a second chromogen for a second time period to generate a second color chromogen-enzyme product, the second color chromogen-enzyme product reflecting light observable as second color. A combination of the light observable as the first color and the light observable as the second color may be observable as a third color, and each color may describe a different analyte density in the biological sample.

Method for labeling concentration density differentials of an analyte in a biological sample

The present disclosure relates methods of chromogen layering, wherein a first chromogen and a first stain (color) are produced on a sample, specific for a first analyte, followed by a second chromogen and a second stain (color) being produced on the same sample, specific for a second analyte. In addition, if desired, by overlaying the second stain on top of the first stain, a unique third color is produced that is specific for a third analyte. Therefore, the distribution of different colors throughout the sample could be used to identify at least two or more analytes simultaneously within a single sample.

Chromogen layering for color generation

Disclosed are methods for treating a biological sample for an analyte-staining assay. These methods are conducted with the use of a single aqueous multi-functional composition for the purposes of performing the steps of deparaffinization, rehydration, antigen retrieval and endogenous enzyme blocking simultaneously optionally at an elevated temperature. These methods are alternatively conducted with use of a single aqueous multi-functional composition that contains a thermostable protease for the purpose of deparaffinization, rehydration, antigen retrieval and endogenous enzyme blocking at an elevated temperature.

Method of pretreatment of biological samples for an analyte-staining assay method

A sample processing system that may be automated and methods are disclosed where samples are arranged on a carrier element and a process operation control system automatically processes the samples perhaps robotically with an operationally-influential exteriorly-consequential information monitor or a data capture element. Significant process details as well as operationally-influential exteriorly-consequential information may be monitored and an automatic notice element may cause notification of a person at some display that may be remote. Various people may be notified, such as an administrator, a supplier, or a manufacturer of an opportunity for some action such as reagent reordering or the like. A simulated motion display may be included to 'watch' simulated operation in real time or long after completion of the actual processing.

Information notification sample processing system and methods of biological slide processing

A sample processing systemthat may be automated and methods are disclosed where a number of sample processing systems, such as stainer, may be connected to a number of separate full function computersthrough a stainer networkthat may be isolated from other communication traffic. A network configuration may permit scalability and addressability so that additional sample processing systems, additional separate full function computers, and additional other devices such as label printersmay be easily added to the system. One or more remote information linksmay be provided so that information transfer on a continuous or perhaps constant basis can be accommodated.

Isolated communication sample processing system and methods of biological slide processing

The present invention concerns an apparatus for staining tissue samples, said apparatus including a reagent section or reagent containers; at least one staining section or tissue samples, a robotic head or robotic element that may move reagent to a predetermined tissue sample, said robotic element being moveable above the reagent and the staining sections, a control element that may manage a staining process, a 2-D optical sensor to detect two-dimensional image data of a relevant property and that can feed the captured image data to the control element. By providing the robotic element with a 2-D optical sensor, a common image processor may be provided having multiple functions. By using a 2-D optical image processing system, the control system of the apparatus may easily be adapted to read various types of data presentations, just as actual images for sections of the apparatus may be identified in order to assess the condition of the apparatus. The optical sensor may be used to automatically identify the slides and the reagent containers present in the apparatus, just as the optical sensor may be used for checking if a slide is misplaced at or absent from a slide position, etc.

Method and apparatus for automatic staining of tissue samples

Systems and methods in accordance with embodiments of the present invention allow for the automatic control and scheduling of a staining apparatus for biological samples on slides present within the apparatus. In some embodiments, the actions of a robot coupled to the staining apparatus, which performs some of the staining tasks on the individual slides in accordance with their respective protocols, may be prioritized and scheduled. In some embodiments, the scheduling may result in increasing or maximizing the throughput of slides. In some embodiments, robot scheduling ensures that the individual slides are processed substantially within the tolerances specified by their respective protocols. In some embodiments, the robot scheduler may respond to spontaneous user actions and adaptively schedule or re-schedule robot actions.

Systems and methods for the automated pre-treatment and processing of biological samples

Systems and methods of sample processing and temperature control are disclosed. The invention may especially relate to temperature control, and may in some embodiments be methods of temperature control of an automated sample processing system and methods of automated sample processing. Specifically, the present invention provides temperature control in relation to sample processing systems and methods of processing samples, and in some embodiments provides temperature control in relation to sample carriers and processing materials such as reagents. Corresponding systems and devices are disclosed, including sample processing systems (), sample carrier temperature regulation systems (), reagent temperature regulation systems, sample processing control systems, and temperature regulation devices, among other embodiments. Scientific fields to which the present invention may have particular applicability include immunohistochemistry, hybridization, fluorescent in-situ hybridization, special staining, such as special staining of histological samples, microarray sample processing, and cytology, as well as potentially other chemical and biological applications.

Systems and methods of sample processing and temperature control

A sample processing systemthat may be automated and methods are disclosed where sample(s)are arranged on a carrier elementand a process operation control systemautomatically processes the sample(s) perhaps robotically according to an desired aggregation of event dictated by an input. Alteration of an initial aggregated event topology may be accepted while the system is processing an initial aggregation and varied-parameter robotic control simulation functionalitiesmay be accomplished to determine an enhanced sequence for processing. Suggested operator actions may be displayed that might further enhance the scheduling of the altered aggregated event topology together with an automatic operator need promptthat may inform an operator of a need for a particular action in order to accomplish the desired tasks. Reversibility to proposed changes may be made available so that an operator may avoid having to activate proposed changes if they cause a processing result that is not acceptable.

Growing community of inventors

Ojal, CA, United States of America

Marc E Key