Northfield Laboratories, Inc.

Haemonetics Corporation

Other

University of Illinois

Hemoglobin Oxygen Therapeutics LLC

Marc Doubleday

Richard E DeWoskin

Anthony Avella

Robert McGinnis

Gabriel Chavez

Fowzia S Zaman

Steven A Gould

Jose Oberholzer

George Hides

Robert L Mcginnis

Richard Dewoskin

In some embodiments, the invention provides methods for detecting and/or classifying an anticoagulant at a therapeutically relevant amount or higher in a patient, including subjecting a sample of a control blood component (known not to contain the anticoagulant) to a clotting assay in the presence of a Factor Xa reagent to obtain a control clotting measurement; and subjecting a sample of a blood component from a patient suspected of having the anticoagulant to the clotting assay in the presence of the Factor Xa reagent to obtain a patient clotting measurement, wherein the patient clotting measurement sample greater than the control clotting measurement indicates the presence of the anticoagulant at a therapeutically relevant amount or higher in the patient. In some embodiments, the invention includes methods for classifying an anticoagulant as an anti-Factor Xa or a direct thrombin inhibitor anticoagulant using a clotting assay in the presence of an ecarin reagent.

Detection and classification of an anticoagulant using a clotting assay

Solutions and suspensions comprising polymerized hemoglobin derived from human blood are disclosed. The solutions and suspensions may comprise cell culture medium, an enzyme (such as a protease), and/or a buffer. Processes of preparing the solutions and suspensions are also disclosed. The solutions and suspensions may be employed in methods of isolating mammalian cells, such as pancreatic islets, methods of preserving mammalian tissue and organs, methods of aiding the recovery of mammalian cells following their isolation, methods of maintaining mammalian cells, methods of propagating mammalian cells, and methods of treating a mammal with diabetes.

Method of isolating cells from a tissue in a mammal

A multi-functional, personal flotation device provides a life preserver for a person in combination with an integrated tethering system to aid in retrieval of the wearer. The integrated tethering system may be used alone or in conjunction with an integrated signaling device. The integrated signaling device extends when the life preserver is use. The integrated tethering system facilitates the recovery of a person wearing the multi-functional, personal flotation device. The integrated signaling device or the integrated tethering system may also include a sound or a visual alarm.

Multi-functional, personal flotation device

A process is disclosed for the preparation of an essentially tetramer-free, substantially stroma-free, polymerized, pyridoxylated hemoglobin. Also disclosed is an essentially tetramer-free, substantially stroma-free, polymerized, pyridoxylated hemoglobin product capable of being infused into human patients in an amount of up to about 5 liters.

Method and apparatus for preparing an acellular red blood cell substitute

A hemoglobin solution packaged in a flexible oxygen-impermeable container system. The container system includes a multi-layer film having at least a product contact layer, an oxygen and moisture barrier layer and an exterior layer. The flexible container system further includes an interface port for filling the flexible container with the hemoglobin solution and delivering the hemoglobin solution. The hemoglobin solution comprises a substantially stroma and tetramer free, cross linked, pyridoxylated hemoglobin solution including preservatives such as ascorbic acid, glycine and dextrose.

Stabilized hemoglobin solutions

A substantially tetramer free hemoglobin solution and a method for producing a substantially tetramer free hemoglobin solution. The method includes polymerizing a solution of hemoglobin, treating the polymerized hemoglobin solution to partially degrade the polymer to tetramer and removing tetramer from the hemoglobin solution.

Polymerized hemoglobin solutions having reduced amounts of tetramer and method for preparing

Methods for treating a mammal suffering from massive blood loss comprising administering to the mammal a polymerized hemoglobin solution.

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Cary, IL, United States of America

Marc Doubleday