Iowa State University Research Foundation Inc

University of Utah Research Foundation

Arizona State University

The United States of America As Represented by the Department of Agriculture/cooperative State Research Education and Extension Service (usda/csrees)

Marc D Porter

Robert J Lipert

Ruth Shinar

Jing Ni

G Brent Dawson

Michael C Granger

Vivian Wynne Jones

Mark A Hayes

Mark Christopher Tondra

Eric R Henderson

Curtis L Mosher

Jennifer Harnisch Granger

Guojun Liu

Duane E Weisshaar

Chuan-Jian Zhong

John P Bannantine

Mikhail Skliar

David K Hoffman

Betsy Jean Yakes

John-Bruce DeVault Green

Robert T Doyle

Desiree S Grubisha

Salma Rahman

Lai-Kwan Chau

Thomas P Jones

Shelley J Coldiron

Alexis Ramirez

Jooneon Park

Robert Joseph Soto

Samuel Hayes

Brennan Copp

The present disclosure provides systems and methods for separating one or more analytes within a fluid mixture, and characterizing and/or detecting properties associated with the one or more analytes. In some embodiments, the systems provided herein contain a dielectrophoresis device, such as a gradient insulator-based dielectrophoresis device (g-iDEP). The present disclosure provides systems and methods for separating and characterizing analytes using particle or nanoparticle tracking analysis (NTA). NTA offers various advantages because, particle size and concentration can be calculated in real time, allowing label-free and simultaneous characterization and separation of samples with mixed and unknown analytes.

Systems and methods for non-destructive isolation, concentration, and detection for unbiased characterization of nano- and bioparticles

This invention discloses a design a capillary column for use with electrochemically modulated liquid chromatography (EMLC). The capillary design, which results in a marked reduction in the flow of current through the column, enables the use of a two-electrode column construction that overcomes the mechanical and electrical shortfalls of the conventional standard bore design.

Two-electrode configuration for separations based on electrosorption in electrochemically modulated liquid chromatography (EMLC)

Discrete magnetic nanoparticles synthesized using a layer-by-layer technique are disclosed. The nanoparticles contain a magnetic core having a large magnetic moment, a plurality of layers and an exterior coating. The nanoparticles have utility in a wide range of biological and bioanalytical applications.

Discrete magnetic nanoparticles

The invention provides nanoparticles and nanoparticle conjugates comprising one or more redox-active species, methods of making nanoparticles and nanoparticle conjugates, and methods for using nanoparticles and nanoparticle conjugates, for example, as diagnostic agents for the detection of various analytes.

Redox polymer nanoparticles

The present invention provides a new class of Raman-active reagents for use in biological and other applications, as well as methods and kits for their use and manufacture. Each reagent includes a Raman-active reporter molecule, a binding molecule, and a surface enhancing particle capable of causing surface enhanced Raman scattering (SERS). The Raman-active reporter molecule and the binding molecule are affixed to the particle to give both a strong SERS signal and to provide biological functionality, i.e. antigen or drug recognition. The Raman-active reagents can function as an alternative to fluorescence-labeled reagents, with advantages in detection including signal stability, sensitivity, and the ability to simultaneously detect several biological materials. The Raman-active reagents also have a wide range of applications, especially in clinical fields (e.g., immunoassays, imaging, and drug screening).

Raman-active reagents and the use thereof

A process of detection of the causative agent of Johne's disease (subsp.) (MAP) by detecting shedding of surface protein of MAP. A preferred way is use of surface enhanced Raman Spectroscopy. The system of detecting MAP shedding of protein provides early detection and diagnosis, and therefore allows early treatment for Johne's disease in ruminant animals.

Bioamplification for microbial sensor signal transduction

A system and method for detecting a detectably labeled cell or component thereof in a sample comprising one or more cells or components thereof, at least one cell or component thereof of which is detectably labeled with at least two detectable labels. In one embodiment, the method comprises: (i) introducing the sample into one or more flow cells of a flow cytometer, (ii) irradiating the sample with one or more light sources that are absorbed by the at least two detectable labels, the absorption of which is to be detected, and (iii) detecting simultaneously the absorption of light by the at least two detectable labels on the detectably labeled cell or component thereof with an array of photomultiplier tubes, which are operably linked to two or more filters that selectively transmit detectable emissions from the at least two detectable labels.

System and method for detecting cells or components thereof

The invention provides nanoparticles comprising one or more redox-active species, methods of making such nanoparticles, and methods for using such nanoparticles, for example, as diagnostic agents for the detection of various analytes.

Method and apparatus for manipulating and monitoring analyte flowing in fluid streams. A giant magnetoresistive sensor has an array of sensing elements that produce electrical output signals which vary in dependence on changes in the magnetic field proximate the sensing elements. The analyte is included in a stream, such that the stream has a magnetic property which is dependent on the concentration and distribution on the analyte therein. The stream is flowed past the giant magnetoresistive sensor and in sufficiently close proximity to cause the magnetic properties of the stream to produce electrical output signals. The electrical output signals are monitored as an indicator of analyte concentration or distribution in the stream flowing past the GMR sensor. Changes in the magnetic field produced by the background stream are introduced by analyte molecules, whose presence in the flow past the GMR will effect the output reading.

Method and apparatus for magnetoresistive monitoring of analytes in flow streams

An acoustic wave based-chemical sensor utilizing a crystal substrate and a coating of at least two blended materials is disclosed. The blended materials comprise a combination of (a) a high glass transition temperature polymer or a material of high melting point, and (b) a low glass transition temperature polymer or a material having a low melting point. Transducers are connected to the crystal substrate to generate an alternating potential across the crystal substrate, which in turn causes the crystal to resonate due to the converse piezoelectric effect. The blended coating absorbs the analyte, thus changing the mass of the chemical sensor, and accordingly changing its resonant frequency. The transducers detect this change in resonant frequency to indicate that the analyte is present. The use of blended materials results in a thicker coating combining the preferred properties of the blend constituents, such as improved detection sensitivities, faster response times, less acoustic wave damping, and higher operational temperature ranges.

Chemical sensor and coating for same

An acoustic wave based-chemical sensor containing a crystal substrate and a coating of small particulate matter is disclosed. The small particulate matter can be graphite particles. Transducers are connected to the crystal substrate to generate an alternating potential across the crystal substrate, which in turn causes the crystal to resonate due to the converse piezoelectric effect. The coating absorbs the analyte, thus changing the mass of the sensor, and accordingly changing its resonant frequency. The transducers detect this change in resonant frequency to indicate that the analyte is present. The use of small particulate matter results in a coating having a large surface area which facilitates mass uptake of large amounts of VOCs, improved acoustic properties even with relatively thick coatings, and a high operational temperature range.

A height referencing biochemical cassette comprising of a surface suitable to act as a coupling agent, a height referencing indicator, and molecules bonded to the surface. This cassette is a capable of use to test for bonding to these molecules through measuring the height difference between the indicator and the surface. This invention provides an efficient means to quickly and easily test for bonding of molecules to other molecules.

Height referencing biochemical cassette

An electrochemical method for forming a partial monomolecular layer of a predetermined extent of coverage of a thiolate of the formula, XRS--, therein R can be a linear or branched chain hydrocarbon or an aromatic or the like and X can be any compatible end group, e.g., OH, COOH, CH.sub.3 or the like, upon a substrate such as gold, which involves applying in an electrochemical system a constant voltage preselected to yield the desired predetermined extent of coverage.

Growing community of inventors

Ames, IA, United States of America

Marc D Porter