Other

The Salk Institute for Biological Studies

Ixsys, Incorporated

Cistem Molecular Corporation

Marc Charles Ballivet

Stuart Alan Kauffman

Stephen Fox Heinemann

James Richard Boulter

James Warner Patrick

Evan Samuel Deneris

Robert M Duvoisin

Eden Deer Heinemann

Keiji Wada

Daniel Jay Goldman

John Gerard Connolly

The present invention relates to a family of neuronal nicotinic acetylcholine receptors comprised of neuronal agonist and non-agonist binding subunits, and DNA sequences encoding such subunits. These novel neuronal nicotinic acetylcholine receptor subunits include the agonist binding subunits alpha2, alpha3, alpha4, and alpha5, plus non-agonist binding subunits beta2, beta3 and beta4. Representative cDNA clones that contain the DNA sequences of the invention have been deposited with the American Type Culture Collection for patent purposes.

Neuronal nicotinic acetylcholine receptor compositions

The present invention is directed to a process for the production of a peptide, polypeptide, or protein having a predetermined property. In accordance with one embodiment, the process begins by producing by way of synthetic polynucleotide coupling, stochastically generated polynucleotide sequences. A library of expression vectors containing such stochastically generated polynucleotide sequences is formed. Next, host cells containing the vectors are cultured so as to produce peptides, polypeptides, or proteins encoded by the stochastically generated polynucleotide sequences. Screening or selection is carried out on such host cells to identify a peptide, polypeptide, or protein produced by the host cells which has the predetermined property. The stochastically generated polynucleotide sequence which encodes the identified peptide, polypeptide, or protein is then isolated and used to produce the peptide, polypeptide, or protein having the predetermined property.

Process for obtaining DNA, RNA, peptides, polypeptides, or protein by recombinant DNA technique

Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique

The invention provides a method of identifying nucleic acid molecules that contain cis acting nucleic acid elements. Also provided is a method of isolating nucleic acid binding factors. The invention also provides methods of identifying compounds that are cis acting nucleic acid element analogs, compounds that are nucleic acid binding factor analogs, compounds that selectively bind cis acting nucleic acid elements and compounds that selectively displace binding between a nucleic acid binding factor and a cis acting nucleic acid element or between nucleic acid binding factors. Also provided is a method of determining a binding state of a nucleic acid. Pluralities of isolated nucleic acid molecules containing cis acting nucleic acid elements, of isolated cis acting nucleic acid elements and of isolated nucleic acid binding factors are also provided. The invention further provides methods of treating pathological conditions using molecules of the invention to alter genetic activities of nucleic acids involved in pathological conditions.

Methods and compositions for identifying nucleic acids containing cis acting elements

Neuronal nicotinic acetylcholihne receptor compositions

Method of identifying cis acting nucleic acid elements

Process for obtaining DNA, RNA, peptides, polypeptides, or proteins, by

Process for the production of expression vectors comprising at least one

Process for the production of stochastically-generated

Process for the production of stochastically-generated transcription or

Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by

Method of identifying a stochastically-generated peptide, polypeptide,

Neuronal nicotinic acetylcholine receptor assay

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Geneva, Switzerland

Marc Charles Ballivet