Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Japan Tobacco Inc.

Pioneer Hi-bred, Inc.

Pioneer Hi-bred Int'l Inc.

Centro De Investigacion Y De Estudios Avanzados Del Instituto Politecnico Nacional (cinvestav)

Washington State University

Unversität Zürich

Corteva Agriscience LLC

Marc C Albertsen

Tim Fox

Mary Trimnell

Gary A Huffman

Andrew Mark Cigan

Yongzhong Wu

Howard P Hershey

John A Howard

Bailin Li

Larry R Beach

Keith S Lowe

Carl W Garnaat

Manjit Singh

Bradley Allen Roth

Marianna Faller

Ryan Whitford

Ute Baumann

Catherine D Brooke

Youngzhon Wu

Elise Tucker

Michael W Lassner

April L Leonard

John Howard

Hajime Sakai

Yoshimitsu Takakura

Stanley Luck

Scott V Tingey

Yukoh Hiei

Mark E Williams

Toshihiko Komari

Timothy W Fox

Ichiro Oka

Satoru Usami

Toshiyuki Komori

Teruyuki Imayama

Masakazu Kashihara

Norio Kato

Timmy L Kendall

Brian R Loveland

William James Gordon-Kamm

Bo Shen

Steven J Corak

Dorothy A Pierce

Shai J Lawit

Sheila E Maddock

Michelle Van Allen

David D Songstad

Gary A Huffma

Marissa Simon

Loverine Taylor

Jaime Padilla Calzada

Isaac Rodriguez Arevalo

Nidia Sanchez Leon

Gloria Leon Martinez

Elvira Hernandez Lagana

Carlos Gonzalez Chavez

Edgar Demesa Arevalo

Carlos Barragan Rosillo

Jean-Philippe Vielle Calzada

Yongzhon Wu

Daniel Rodriguez Leal

Tim W Fox

Mark A Chamberlin

Joana Bernardes De Asis

Gion Arco Brunner

Frederique Pasquer

Nina Chumak

Ueli Grossniklaus

Compositions and methods for modulating male fertility in a plant are provided. Compositions comprise nucleotide sequences, and active fragments and variants thereof, which modulate male fertility. Further provided are expression cassettes comprising the male fertility polynucleotides, or active fragments or variants thereof, operably linked to a promoter, wherein expression of the polynucleotides modulates the male fertility of a plant. Various methods are provided wherein the level and/or activity of the sequences that influence male fertility is modulated in a plant or plant part.

Compositions and methods comprising male fertility sequences

Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and/or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.

Wheat MS1 polynucleotides, polypeptides, and methods of use

Provided herein are methods and compositions for restoring fertility and maintaining sterility in plants. In particular, one method disclosed includes introducing into a male-sterile plant, wherein the plant comprises one or more homozygous mutations in a male-fertility gene, a plant restoration donor chromosomal component comprising a plant-derived polynucleotide that confers a plant phenotypic marker linked to a male-fertility restoration locus that functionally complements the male-sterility phenotype from the one or more homozygous mutations in the male-sterile plant. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker and the male-fertility restoration locus are linked to each other and located on the same chromosomal arm on the plant restoration donor chromosomal component. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker, the male-fertility restoration locus, or both are modified.

Fertility restoration in plants

Methods and compositions useful for avoiding plant meiosis and clonal reproduction through seed are provided herein. The present disclosure provides polynucleotides and related polypeptides of Spo11, Rec8, OSD1-1A, and OSD1-3A and methods and compositions for suppressing their expression level or activity.

Methods and compositions for producing clonal, non-reduced, non-recombined gametes

Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably-linked gene. Mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations may be tracked. Male-sterile plants may be maintained by crossing with a maintainer plant. Hybrids can be produced using the male-sterile plants.

Methods for identifying and/or selecting wheat plants having a mutation in an endogenous Ms1 gene

Disclosed are methods of obtaining clonal seeds, methods of plant cloning, methods of screening for maternal plants that produce clonal seeds asexually and methods of increasing yield of clonal seeds. Also disclosed are constructs comprising a nucleic acid that may silence the activity of a RNA-dependent DNA methylation pathway gene. Further disclosed are maternal plants comprising a construct wherein the construct comprises an exogenous nucleic acid sequence, wherein the construct renders the maternal plant defective for RNA-dependent DNA methylation.

Methods for reproducing plants asexually and compositions thereof

A nuclear maize gene is mutated to result in dominant male sterility. Two mutants are provided which impact signal peptide processing. Restoration constructs and methods are provided for use of the dominant male-sterile mutants in producing hybrid seed for male-sterile hybrid plants. Other signal-peptide modifications are provided. Orthologs of the wild type or mutated gene in various species are provided.

Genetic reduction of male fertility in plants

Nucleotide sequences of a Msca1 gene, critical to male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in impacting male fertility in plants.

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Genetic male sterile plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production.

Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.

Drought tolerant plants and related constructs and methods involving genes encoding DTP21 polypeptides

Growing community of inventors

Grimes, IA, United States of America

Marc C Albertsen