Roquette Freres

Marc Biguet

Pierre Lanos

Fabrice Allain

Mathieu Carpentier

Agnès Denys

Héla Hacine-Gherbi

Roselyne Bernard

Pierrick Duflot

Stéphane Bourdain

Stephane Bourdain

The present invention relates to a biological method for the dosage of peptidoglycans in a sample, especially a sample of glucose polymers.

Biological dosage of peptidoglycans

The present invention relates to a biological method for assaying peptidoglycans (PGN) in a sample, particularly a sample of glucose polymers. The PGN assay includes: a) treating the glucose polymer sample by sonication, heating, and/or alkalizing; b) placing the treated sample or a dilution thereof in contact with a recombinant cell expressing an exogenous TLR2 (toll-like receptor 2) and a reporter gene directly dependent on the signaling pathway associated with the TLR2. The reporter gene codes for a colored or fluorescent protein or for a protein the activity of which is measurable with or without a substrate; c) measuring the reporter gene signal; and d) determining the amount of PGN in the sample using a standard curve of the correlation between the amount or PGN and the strength of the reporter gene signal.

Biological assay of peptidoglycans

The invention relates to a method of purifying glucose polymers for the production of peritoneal dialysis solutions, characterized in that it includes at least one step of processing activated carbon and/or granular black, at least one sterilizing filtration step, at least one heat treatment step, and at least one ultrafiltration step.

Method for purifying glucose polymers for peritoneal dialysis solutions

Growing community of inventors

Neuve Chapelle, France

Marc Biguet