Danisco US Inc.

Marc Anton Bernhard Kolkman

David Aaron Estell

Frank Wouter Koopman

Chris Leeflang

Richard Ray Bott

Frits Goedegebuur

Jian Yao

Hongxian He

Lilia Maria Babe

Rie Mejldal

Ryan L Frisch

Katherine Augustyn

Luis G Cascao-pereira

Steven D Doig

Roopa Santosh Ghirnikar

Dennis De Lange

Harm Jan Mulder

Harm Mulder

The present disclosure is generally related to compositions and methods for obtainingcells/strains having increased protein production capabilities. Certain embodiments of the disclosure are related to genetically modifiedcells/strains derived from parentalcells/strains comprising a variant rghR2 gene.

Compositions and methods for increased protein production in

Disclosed are compositions and methods relating to variant maltohexaose-forming alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.

Variant maltohexaose-forming α-amylase variants

The present disclosure provides AprL-clade protease enzymes, including variant AprL-clade protease enzymes, nucleic acids encoding same, and compositions and methods related to the production and use thereof, including an AprL-clade variant subtilisin enzyme that has improved stability and/or soil removal compared to a parent AprL-clade subtilisin enzyme.

AprL-clade protease variants and uses thereof

The instant disclosure is generally related to compositions and methods for obtaining and constructinghost cells (e.g., protein production host cells, cell factories) having increased protein production capabilities. Certain embodiments of the disclosure are directed to efficient genetic modifications ofcells and the subsequent selection of suchcells having increased protein production capabilities. Certain other embodiments of the disclosure are generally related to methods and compositions for producing/obtaining auxotrophiccells, wherein certain other embodiments of the disclosure are directed to methods and compositions for restoring prototrophy in auxotrophiccells, and expressing genes of interest (GOIs) in such restored prototrophycells.

Methods and compositions for efficient genetic modifications ofstrains

Variant maltohexaose-forming alpha-amylase variants

Compositions and methods for increased protein production in bacillus licheniformis

AprL-clade subtilisin protease variants and uses thereof

Growing community of inventors

Oegstgeest, Netherlands

Marc Anton Bernhard Kolkman