Amgen Inc.

Marc A Caporini

Ron C Kelly

Andrew Thomas Parsons

William Powazinik, Iv

Twinkle R Christian

Neill Burt

Laura Nicholson

Rizwan Sharnez

Brian Mcneil Cochran

William Trieu

Brian McNeil Cochran

Mariana De Maillé

Disclosed herein are methods of preparing a composition comprising crystalline biomolecules, for example, crystalline antibodies. In exemplary embodiments, the method comprises forming a fluidized bed of crystalline biomolecules using, for example, a counter-flow centrifuge to exchange buffer and/or to concentrate the crystalline biomolecules in a solution. Also provided are methods of detecting crystalline biomolecules and/or amorphous biomolecules in a sample.

Methods directed to crystalline biomolecules

The present invention relates to an improved, efficient, scalable process to prepare intermediate compounds, such as compound 5M, having the structure useful for the synthesis of compounds that target KRAS G12C mutations, such as

Synthesis of key intermediate of KRAS G12C inhibitor compound

Disclosed herein is a method of conducting direct detectionH solid state NMR ('ssNMR') on a macromolecule-containing solid state formulation. The method includes conducting aH spin-lattice relaxation time (“T”) experiment on the solid state formulation at various temperatures to generate a Tvalue at each temperature, converting the Tvalues toH spin-lattice relaxation rate (“R”), and plotting Rversus temperature to generate a relaxation rate curve for the solid state formulation. The relaxation rate curve can be analyzed to determine the molecular mobility of the macromolecule in the solid state formulation, the degree of aggregation in the solid state formulation, and/or the stability of the solid state formulation.

Method for conducting solid state NMR on macromolecule-containing solid state formulations

The present invention relates to an improved, efficient, scalable process to prepare intermediate compounds, such as compound 5M, having the structure useful for the synthesis of compounds that target KRAS G12C mutations, such as 

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Cambridge, MA, United States of America

Marc A Caporini