Jiangsu Academy of Agricultural Sciences

Guotai (taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou

Maojun Liu

Li Wang

Qiyan Xiong

Zhixin Feng

Yuzi Wu

Yanna Wei

Yuan Gan

Beibei Liu

Guoqing Shao

Yanfei Yu

Yun Bai

Jia Wang

Daohua Zhang

Dongxia Liu

Lizhong Hua

Fangfang Bai

Haiyan Wang

Guoqinq Shao

A composition and a kit for detectingare provided. The composition for detectingis an aqueous solution including a primer M-F, a primer M-R, and a probe M-P. A sequence of the M-F is shown in SEQ ID NO: 1. A sequence of the M-R is shown in SEQ ID NO: 2. A nucleotide sequence of the probe M-P is shown in SEQ ID NO: 3, and includes a fluorophore FAM linked at a 5' terminus and a quencher BHQ1 linked at a 3′ terminus. The composition exhibits high sensitivity, strong specificity, and a wide detection range when used in the detection of

Composition and kit for detecting mycoplasma

Disclosed are an attenuated live vaccine against mycoplasmal pneumonia of swine (MPS) and use thereof. In the present invention, pathological lung tissues of swine having typical(Mhp) infection and no obvious other pathogenic infections are screened, and subcultured 100 generations in lungs of newborn rabbits; then, Mhp strains are isolated and serially subcultured in a medium; and the Mhp strain AN306 is obtained by screening a plurality of strains, which is deposited with an accession number: CCTCC NO. M2012431. Also disclosed is a live vaccine formulation against MPS prepared on the basis of the attenuated strain and comprising live attenuated strain, a pharmaceutically acceptable carrier or excipient, and optionally an adjuvant and immunogens of other pathogens.

Attenuated live vaccine against mycoplasmal pneumonia of swine (MPS) and use thereof

Growing community of inventors

Nanjing, China

Maojun Liu