Politecnico Di Milano

Consiglio Nazionale Delle Ricerche

Universitá Degli Studi Di Milano-Bicocca

Fondazione Cariplo

Istituto Ortopedico Galeazzi S.p.a

Fondazione Cassa Di Risparmio Delle Province Lombarde

Manuela Teresa Raimondi

Roberto Osellame

Giulio Cerullo

Matteo Lagana

Paola Petrini

Carmen Giordano

Roberto Giacometti

Diego Albani

Tommaso Zandrini

Claudio Conci

Giuseppe Chirico

Andrea Remuzzi

Luca Izzo

Matteo Lagana'

Arianna Colombini

A millifluidic device for cultures of biological agents includes a main body having a first hole closed at the bottom, a separator a membrane fixed to the separator, and a plug closing the first hole. The separator is designed to be placed in the first hole and is extractable from the first hole. The membrane divides the first hole into an upper half-chamber and a lower half-chamber. A pair of tubes perfuse the lower half-chamber and another pair of tubes perfuse the upper half-chamber. A first slide is placed centrally on the plug and a second slide is placed centrally on the first hole. A cylindrical body rises from the first hole and the second slide is placed on the top of the cylindrical body. The cylindrical body has a second hole, coaxial to the cylindrical body.

Millifluidic device for advanced cultures of biological agents

An implantable medical device obtained by means of two-photon laser polymerisation of a resin to form a three-dimensional matrix, wherein: said three-dimensional matrix comprises a number of levels distributed in height; and said three-dimensional matrix comprises reference means designed to uniquely identify the height of each level from a pre-set reference, by means of a multi-photon fluorescence-excitation microscope; said implantable medical device being characterised in that said reference means comprise a solid having a cross section that varies with height.

Implantable medical device

A device for cell culture comprising: a main body (); said main body () comprises a plurality of circular portions (-); said device comprises a plurality of caps (-); each of said plurality of caps (-) comprises a base structure () having a circular hole (), housing an upper slide () and an elastomeric layer () secured to said slide (); said elastomeric layer () has a rectangular hole (); said plurality of caps (-) being adapted to cooperate with said plurality of circular portions (-); each of said plurality of circular portions (-) each comprise an inlet hole (-) and an outlet hole (-) aligned with the long side of said rectangular hole (), to perfuse the culture chamber (-) located in said rectangular hole () of said elastomeric layer ().

Device for cell culture

The present invention relates to a supermatrix of synthetic niches comprising at least two matrices of niches wherein each matrix comprises n&#xd7;m niches and wherein the distance (d) between a matrix and the other is greater than zero and wherein in each matrix every synthetic niche has one or more walls in common with the others synthetic niches of the matrix. A support for the culture of cells that includes at least one supermatrix and the use of said supermatrix or support for the in vitro culture of cells, in particular stem cells, or for the in vivo implant in a subject in order to promote tissue or organ regeneration through cell repopulation are also within the scope of the present invention.

Synthetic niche matrices for stem cell culture

Growing community of inventors

Milan, Italy

Manuela Teresa Raimondi