Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Centre National De La Recherche Scientifique

Institut National De La Sante Et De La Recherche Medicale

Institut Curie

Université Joseph Fourier - Grenoble 1

Manuel Thery

Jean-Christophe Gabriel

Matthieu Piel

Odile Filhol-Cochet

Yoran Margaron

Remi Galland

Rémi Galland

Laurent Blanchoin

Michel Bornens

Qingzong Tseng

Rajaa Paterski

The present invention relates to a method for predicting the invasive potential of a tumor cell and predicting the efficiency of a treatment. The method is based on the combination of at least three parameters, namely the internuclear distance, the polarization and the cell-cell connectivity.

Method for determining the invasive potential of a tumor cell

The present invention concerns devices and methods for controlling actin filament growth and organization with micropatterned nucleation sites, and their uses for studying actin network formation, for screening of drugs or for preparing complex structures.

Devices and methods for controlling actin filaments growth and organization using micropatterned nucleation sites

The present invention relates to devices and methods for the measurement of cell traction forces. In particular, the invention is based on the use of a soft substrate with cell adhesive micropatterns.

Use of micropatterned soft substrate for measuring of cell traction forces

The present invention relates to a method for preparing three-dimensional actin structures having a well-defined shape and displaying improved mechanical rigidity. This method comprises the steps of (a) providing a polymerization solution comprising actin monomers, a branching agent and a capping agent, (b) providing at least one surface having thereon a pattern which is coated with a nucleating agent, and (c) contacting the at least one surface of step (b) with the polymerization solution of step (a) so as to induce the polymerization of actin and obtain the said desired three-dimensional actin structure. Applications of the present invention in various technological fields such as microelectronics are also provided.

Method for obtaining three-dimensional actin structures and uses thereof

The present invention relates to methods and devices to obtain multicellular arrangements in stable, stationary and reproducible spatial configuration, and optionally with controlled internal cell organization, methods for preparing such devices, methods for studying the cells' shapes, the cells' architectures, the cells' mechanical equilibrium, the cell-cell interaction, the cell movement and migration, the cell differentiation, the global internal cells' organizations, the cells' polarities and division, and/or any function of cells, methods for screening compounds of interest which enhance or inhibit specific cell functions.

Methods and device to constrain multicellular arrangements in stable, stationary and reproducible spatial configuration

The present invention relates to methods and devices for adhering cells in a specific and predetermined position with an adhesive control of internal cell organization, methods for preparing such devices, methods for studying modifications of cell shape and global internal cell organization such as the distribution of cellular compartments, centrosome centering, spindle orientation, internal compartmentalization and internal transports, methods for screening compounds of interest which enhance or inhibit specific cell functions.

Growing community of inventors

Grenoble, France

Manuel Thery