Evolva Sa

Cargill Inc.

Danstar Ferment Ag

Evolva, Inc.

Manuel Quiros Asensio

Veronique Douchin

Simon Carlsen

Jens Houghton-Larsen

Michael Dalgaard Mikkelsen

Swee Chuang Lim Hallwyl

Iben Nordmark Andersen

Francesca Ambri

Helene Lunde Robertsen

Ting Liu Carlson

Arlene M Fosmer

Esben Halkjaer Hansen

Nina Nicoline Rasmussen

Adam Matthew Takos

Jane Dannow Dyekjaer

James C Anderson

Hans Peter Smits

Ariene M Fosmer

Helene Lunde Robertson

The invention relates to recombinant microorganisms and methods for producing steviol glycosides and steviol glycoside precursors.

Production of steviol glycosides in recombinant hosts

Disclosed are methods for producing steviol glycosides, such as rebaudioside D and rebaudioside M, using engineered yeast. In some embodiments, the methods include fermenting with a yeast at a high pH such as of about 5.8 or greater. In some embodiments, the methods can be carried out by first growing the yeast at a lower first pH, and then adjusting the pH to a higher pH.

Fermentation methods for producing steviol glycosides using high pH and compositions obtained therefrom

Growing community of inventors

Soborg, Denmark

Manuel Quiros Asensio