Changshu Institute of Technology

Suzhou Kemanduo Biotechnology Co., Ltd.

Manting Qi

Bin Qi

Limei Wang

Tiantian Xu

Yuhua Wang

Zhenghong Li

Yamei Ji

The invention relates to a GDSL lipase, genetically-engineered bacteria and an application thereof. The GDSL lipase is derived fromCS1801 and its amino acid sequence is as shown in SEQ ID NO.2. After construction of a genetically-engineered bacterium strain, a GDSL lipase is generated through fermentation. Through this enzyme, vitamin A and palmitic acid are converted to produce vitamin A palmitate. The content of the vitamin A palmitate obtained from the conversion is 16.35 mg/L at most. The conversion efficiency is 81.75% at most. This lipase provides a new path to synthesize vitamin A palmitate by the enzymatic method and has an important application prospect.

GDSL lipase, genetically-engineered bacteria and application thereof

The present invention relates to a lipase-producing strain and application thereof. The strain is classified and namedCS1802, with a preservation number of CCTCC NO: M2018262. The strain can be used to produce vitamin A palmitate through whole-cell transformation of vitamin A and palmitic acid. TheCS1802 of the present invention is derived from traditional natural fermented food and is a microorganism generally recognized as safe. The strain can be easily cultured and preserved. The highest content of vitamin A palmitate obtained through whole-cell transformation of vitamin A and palmitic acid is 15.35 mg/L. The highest transformation efficiency is 76.75%. The strain provides a new path for enzymatic synthesis of vitamin A palmitate and has important application prospects.

Strain for producing lipase and application thereof

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Manting Qi