Dsm IP Assets B.v.

Manos Papadakis

The present disclosure discloses the identification and introduction of a specific heterologous gene (denoted as smob), which encodes an α-1,2-fucosyltransferase, into an LNT production strain to produce LNFP-I in particular. The smob gene originates from the organism(https://www.dsmz.de/collection/catalogue/details/culture/DSM-102939), which is a sulfur-oxidizing bacterium isolated from a brackish lake sediment.

Identification of an α-1,2-fucosyltransferase for the in vivo production of pure LNFP-I

This invention relates to a method of producing one or more human milk oligosaccharides (HMOs), in particular LNT and/or LNnT, in a genetically engineered cell comprising an enhanced oligosaccharide transport capability. The genetically modified cell comprises a series of genetic modification which enable the production of one or more HMO(s), and a series of genetic modification that enhances the transport of lactose and produced HMO(s).

Enhancing formation of milk oligosaccharides (HMOs) by modifying lactose import in the cell

The present disclosure relates to the production of sialylated Human Milk Oligosaccharides (HMOs), in particular to the biosynthetic production of 3'-sialyllactose (3′SL), and to genetically engineered cells and methods suitable for said production.

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Hørsholm, Denmark

Manos Papadakis