Global Life Sciences Solutions USA LLC

Ge Healthcare Bio-sciences Ab

Cytiva Bioprocess R&d Ab

Cytiva Sweden Ab

Ge Healthcare Bioprocess R&d Ab

General Electric Company

Manoj Kumar Ramakrishna

Haresh Digambar Patil

Daniel Salomonsson

Klaus Gebauer

Anoop Bhargav

Sebastian John

Praveen Paul

Per Uselius

Per Lofving

Swapnil Puranik

Manish Uddhaorao Choudhary

Pradeep R Kumar

Kishore Chengalvarayan

Nikhil Kamble

Nivedita Phadke

Andreas Castan

Mohammed Mehtab Khan

Shashikanth Agnihotry

Nagaraju Konduru

Sameera Simha Hindupur

Veena Rao

Umesh Pai

Venna Rao

A parallel assembly of chromatography column modules connected in a rigid housing the assembly having one common assembly inlet and one common assembly outlet each column module comprising a bed space filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules in the rigid housing are adapted to connect the bed space of the column module with the assembly inlet and the assembly outlet wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.

Parallel assembly of chromatography column modules

The present invention relates to a computer implemented method for determining a weld integrity test result performed by a system configured to aseptically transferring cells to a container (BR), the method comprising welding () a vial to a connector to obtain a fluid-tight seal between the vial (V) and a connector (C), the connector being provided with a pair of conduits each having one end protruding through the connector and into the vial, sealing () an opposite end of one of the conduits, generating () a fluid pressure different to an ambient fluid pressure at an opposite end of the other conduit, measuring () a fluid pressure within at least one of the conduits, determining () a fluid pressure change based on the ambient pressure and the measured fluid pressure, determining () the weld integrity test result as pass or as fail based on the fluid pressure change.

Method for determining a weld integrity test result

Methods for selecting a filter for a filtration process are described. Input data is received, and based on the input data, one or more filter characteristics for each of a plurality of filter candidates for a filtration process are identified. Process parameters of the filtration process are identified. A computer-implemented simulation process of the filtration process for each of the plurality of filter candidates is performed, based on the identified process parameters and identified filter characteristics. One or more output characteristics of the filtration process are determined based on the simulation. A filter candidate of the plurality of filter candidates is selected based on the one or more output characteristics.

Method, apparatus, system and computer program product for selecting a filter for a filtration process

Cell culture apparatus is disclosed comprising: a cell culture container comprising a flexible tube; a support table; and a pair of opposed holders for holding opposed portions of the tube in a fluid tight manner such that fluid cannot pass through the respective portion inside the tube, the spacing between the said pair being adjustable to provide an adjustable sealed volume in the tube between the holder pair.

Apparatus and methods for adjustable volume cell culture

A parallel assembly () of chromatography column modules () connected in a rigid housing (), the assembly having one common assembly inlet () and one common assembly outlet (), each column module comprising a bed space () filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules in the rigid housing are adapted to connect the bed space () of the column module with the assembly inlet () and the assembly outlet (), wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.

Disclosed is a cell culture system comprising a first cell culture bioreactor system () for culturing cells to a predetermined cell density or quantity, the system including a bioreactor volume (), a process controller (), process control devices () which provide inputs () for the culture volume and culture parameter measurement devices (), wherein the process controller is operable according to plural control program steps to control the process devices to provide inputs for a suitable cell culture environment in the bioreactor volume, and is further operable according to control program steps modified by feedback values from the culture parameter measurement devices, and comprises a memory () operable to record data indicative of the progress of the control program steps. Failure of the system can be rectified by moving the bioreactor volume to another similar system which has access to data indicative of any incomplete program steps which steps may have been modified by feedback from the measurement devices.

Failure recovery in cell culture bioreactors

The present invention relates to a mixing system for a bioreactor, comprising a plurality of supply units (), each being able to hold media for use in a bioreactor a mixing unit () for creating a uniform mixing of media a first feeding mechanism (), arranged to feed media from the supply units () into the mixing unit (), a control unit () operatively connected to the first feeding mechanism () and the mixing unit (), said control unit () being configured to control the first feeding mechanism () to feed predetermined amounts of media from the plurality of supply units () to the mixing unit (), and further being configured to control the mixing unit () to create a uniform mixing of media. The invention also relates to a method for mixing media for use in a bioreactor.

Mixing system

Disclosed is biomanufacturing apparatuscomprising a housingincluding topand bottomfaces which allow stacking of plural housings, an access doorat a front side of the housing, a substantially enclosed bioreactor chamberinside the housing accessible via the door, and a further substantially enclosed regioninside the housing containing electrical parts and/or electronic control components, the chamberincluding: a trayfor supporting a bioreactor, a tray supportincluding a mechanismfor rocking the tray in use; the tray support further including a load cell () to determine changes in the mass load on the tray.

Biomanufacturing apparatus

Disclosed is biomanufacturing apparatuscomprising a housingincluding topand bottomfaces which allow stacking of plural housings, an access doorat a front side of the housing, a substantially enclosed bioreactor chamberinside the housing accessible via the door, and a further substantially enclosed regioninside the housing containing electrical parts and/or electronic control components, the chamberincluding: a trayfor supporting a bioreactor, a tray supportincluding a mechanismfor rocking the tray in use; at least a fluid handling portionof at least one fluid pump device; and at least one connectionfor connecting to a conduit and/or path for carrying fluids, gas and/or electricity.

The present invention relates to a novel bioreactor system for cell cultivation. More specifically, the invention relates to a compact bioreactor system which has several integrated functions and enables small scale static culture as well as scale-up rocking culture in the same bioreactor. The bioreactor system comprises tray for positioning of a cell culture bag having adjustable volume, a lid covering the cell culture bag and provided with heating function, an integrated perfusion unit, an integrated cell loading unit, and an integrated unit for automatic cell culture sampling, wherein the bioreactor system is controlled by a single control unit. The invention also relates to a method of cell culture using the bioreactor system for culture of therapeutic cells.

Bioreactor system for cell cultivation

The present invention provides a device and method for sampling from a cell culture bioreactor. The invention is useful where it is required to determine the cell count, provide a sample for off-line analyses or remove a sample while at the same time retain sterility of the sample for quality control (QC) assessment. The device of the invention allows for facile aseptic sampling at one or more instances in time during a cell expansion in a bioreactor.

Heatseal sampling

A holding system for holding and hoisting a chromatography column of one or more chromatography columns is disclosed. The holding system includes an upright member and a plurality of holding members movably arranged along the upright member. A first supporting member is positioned at an end portion of the chromatography column, wherein a holding member of the plurality of holding members is configured to be engaged to the first supporting member. A second supporting member is positioned at another end portion of the chromatography column. A holding member of the plurality of holding members is configured to be engaged to the second supporting member. One or more hoist assisting members may be also present. A hoist assisting member is operatively connected to the holding member engaged to the first supporting member. The hoist assisting member is operable to move the holding member connected to the first supporting member for hoisting the chromatography column.

Hoisting and holding system for chromatography column

The invention relates to a chromatography column assembly that offers an affordable self-packed column for performing chromatographic separation. The chromatography column assembly comprises a column tube having a first and a second opening at substantially opposite ends, and an end cap of either a snap fit design or a bolted design. The column assembly may further include an adaptor shaft inserted into the column through the end cap for achieving a desired bed volume. The column assembly may also include a second end cap enclosing the second opening, and an optional adaptor shaft may be inserted into the column through the second end cap, enabling further adjustment of the bed volume.

Chromatography column assembly with snap fit end cap and adaptor shaft

The invention discloses an apparatus for aligning and supporting a first and a second length of thermoplastic tubing, preparatory to and during cutting and welding of said lengths of tubing, that includes a first holder arranged to accommodate portions of the first and second lengths of tubing in a first and a second elongated holding space respectively, a second holder arranged to accommodate portions of the first and second lengths of tubing in a third and a fourth elongated holding space respectively, and movable in a direction essentially perpendicular to the holding spaces of the first and second holders from a cutting position, a cutting means arranged between said first and second holders for cutting said lengths of tubing when the second holder is in the cutting position and an aligning means to align these lengths of tubing with each other.

Growing community of inventors

Karnataka, India

Manoj Kumar Ramakrishna