Life Technologies Corporation

Applied Biosystems, LLC

Apton Biosystems, Inc.

Pacific Biosciences of California, Inc.

Other

Manohar R Furtado

Rixun N Fang

Jaiprakash G Shewale

Bryan P Staker

Pius Brzoska

Windsor Owens

Niandong Liu

Maxim G Brevnov

Norman Burns

Hemant S Pawar

Olga Petrauskene

Michael Malicdem

Maura Barbisin

Cristin O'Shea

Craig Cummings

Paolo Vatta

Lily Wong

Kevin Hacker

Gregory Govoni

Nikolay Sergeev

Johnie Young

Melissa Barker

Mariela Cuadras

James C Nurse

Elena Bolchakova

Robert Tebbs

Jen-Kuei Liu

Jim Nurse

Michael Brewer

Dennis Ballinger

Somaya Bit

Rixun Fang

Sueh-Ning Liew

Elena Bolchacova

Manfred Kayser

Kaye Ballantyne

Craig A Cummings

Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

Methods and kits for extraction of DNA

Disclosed herein are methods and systems for detection and discrimination of optical signals from a densely packed substrate. There have broad applications for biomolecule detection near or below the diffraction limit of optical systems, including in improving the efficiency and accuracy or polynucleotide sequencing applications.

Sequencing and high resolution imaging

Disclosed herein are methods and systems for detection and discrimination of optical signals from a densely packed substrate. These have broad applications for biomolecule detection near or below the diffraction limit of optical systems, including in improving the efficiency and accuracy of polynucleotide sequencing applications.

Densley-packed analyte layers and detection methods

The methods and compositions provided herein relate to the discovery of 13 STR markers, found on the human Y chromosome, having surprisingly high mutation rates when compared with 173 other Y-STR markers known today. The set of RM-Y-STRs may overcome the current dilemma of Y-chromosome analysis in forensic applications due to their extraordinary mutation properties. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.

Analysis of Y-chromosome STR markers

Processing high density analyte arrays

Devices and methods for detecting microbial contaminants, such as bacteria and fungi, in fluids such as drinking water, pharmaceutical solutions and tissue culture media are provided. More particularly, provided are filtration devices for capture and processing of microorganisms from fluids, and improved methods for recovery, lysis and detection of microorganisms based on a combination of physical disruption with small beads and lysis solutions.

Device for capture and lysis of microorganisms from liquids and methods of use thereof

Disclosed is a multi-primer amplification assay, method and kits for detectingspecies and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicatesin the sample.

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The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping.

Multiplex compositions and methods for quantification of human nuclear DNA and human male DNA and detection of PCR inhibitors

Device for capture and lysis of microorganisms from liquids

Disclosed are methods and kits for the specific detection ofO157:H7 and notO55:H7 from samples such as: complex food matrices, water, beverages, fermentation broths, forensic & biological samples, and environmental samples including food processing and manufacturing surfaces. In some embodiments, a method of the disclosure comprises: hybridizing at least a first pair of polynucleotide primers to at least a first target polynucleotide sequence, hybridizing at least a second pair of polynucleotide primers to at least a second target polynucleotide sequence, amplifying the at least first and at least second target polynucleotide sequences, and detecting the first and second amplified target polynucleotide sequence products, wherein the detection of both the first amplified target polynucleotide sequence product and the second amplified target polynucleotide sequence product is indicative of the presence ofO157:H7 in a sample and notO55:H7.

Growing community of inventors

San Ramon, CA, United States of America

Manohar R Furtado